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Three-Dimensional Printed Target Plates for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
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SYSNO ASEP 0535928 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Three-Dimensional Printed Target Plates for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Tvůrce(i) Fialová, Jana (MBU-M) ORCID
Hrabák, J. (CZ)
Studentová, V. (CZ)
Kavan, Daniel (MBU-M) RID, ORCID
Pompach, Petr (MBU-M) RID, ORCID
Novák, Petr (MBU-M) RID, ORCIDZdroj.dok. Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 92, č. 19 (2020), s. 12783-12788Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova MALDI-TOF MS Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NV19-05-00541 GA MZd - Ministerstvo zdravotnictví EG17_107/0011449 GA MPO - Ministerstvo průmyslu a obchodu Výzkumná infrastruktura CIISB II - 90127 - Masarykova univerzita Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000580426800008 EID SCOPUS 85095970297 DOI 10.1021/acs.analchem.0c01737 Anotace Three-dimensional printing (3D printing) is a fast-growing technology with high impact in industry, medicine, and the life sciences. Fused deposition modeling (FDM), which uses plastic filaments extruded through a heated nozzle, is the most common 3D printing technology for creation of objects. In this work, the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) target plates printed by FDM technology using conductive plastic material were evaluated for their detection capability of proteins and peptides. The 3D printed MALDI targets were validated by analysis of different types of bacteria and compared with commercially available MBT BioTargets. The results indicate that 3D printed MALDI targets are comparable to standard MBT BioTargets and stainless-steel targets and may be used for different MALDI-TOF MS applications. The 3D printing allows easy manufacturing of MALDI targets with different dimensions and spot geometry. Moreover, the 3D printed MALDI targets are disposable, cheap, and easy to produce. These features make them a suitable cost-effective alternative to conventional targets for any MALDI MS analysis. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01737
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