Počet záznamů: 1
Cytogenetic Characterization of Seven Novel satDNA Markers in Two Species of Spined Loaches (Cobitis) and Their Clonal Hybrids
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SYSNO ASEP 0531710 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cytogenetic Characterization of Seven Novel satDNA Markers in Two Species of Spined Loaches (Cobitis) and Their Clonal Hybrids Tvůrce(i) Marta, Anatolie (UZFG-Y) ORCID
Didukh, Dmitrij (UZFG-Y) ORCID
Bartoš, Oldřich (UZFG-Y)
Majtánová, Zuzana (UZFG-Y) RID, ORCID
Janko, Karel (UZFG-Y) RID, ORCIDČíslo článku 647 Zdroj.dok. Genes. - : MDPI
Roč. 11, č. 6 (2020)Poč.str. 16 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova clonal vertebrates ; hybridization ; satellite DNA ; FISH ; mitotic and lampbrush chromosomes Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology CEP GA19-21552S GA ČR - Grantová agentura ČR EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000550919700001 EID SCOPUS 85086168899 DOI 10.3390/genes11060617 Anotace Interspecific hybridization is a powerful evolutionary force. However, the investigation of hybrids requires the application of methodologies that provide efficient and indubitable identification of both parental subgenomes in hybrid individuals. Repetitive DNA, and especially the satellite DNA sequences (satDNA), can rapidly diverge even between closely related species, hence providing a useful tool for cytogenetic investigations of hybrids. Recent progress in whole-genome sequencing (WGS) offers unprecedented possibilities for the development of new tools for species determination, including identification of species-specific satDNA markers. In this study, we focused on spined loaches (Cobitis, Teleostei), a group of fishes with frequent interspecific hybridization. Using the WGS of one species,C. elongatoides, we identified seven satDNA markers, which were mapped by fluorescence in situ hybridization on mitotic and lampbrush chromosomes ofC. elongatoides,C. taeniaand their triploid hybrids (C. elongatoidesx 2C. taenia). Two of these markers were chromosome-specific in both species, one had centromeric localization in multiple chromosomes and four had variable patterns between tested species. Our study provided a novel set of cytogenetic markers forCobitisspecies and demonstrated that NGS-based development of satDNA cytogenetic markers may provide a very efficient and easy tool for the investigation of hybrid genomes, cell ploidy, and karyotype evolution. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/2073-4425/11/6/617
Počet záznamů: 1