Online Concentration of Bacteria from Tens of Microliter Sample Volumes in Roughened Fused Silica Capillary with Subsequent Analysis by Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

0524062 - ÚIACH 2021 RIV US eng J - Článek v odborném periodiku
Horká, Marie - Šalplachta, Jiří - Karásek, Pavel - Růžička, F. - Roth, Michal
Online Concentration of Bacteria from Tens of Microliter Sample Volumes in Roughened Fused Silica Capillary with Subsequent Analysis by Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.
ACS Infectious Diseases. Roč. 6, č. 3 (2020), s. 355-365. ISSN 2373-8227
Grant CEP: GA ČR(CZ) GA19-00742S; GA MZd(CZ) NV16-29916A; GA MV VI20172020069
Institucionální podpora: RVO:68081715
Klíčová slova: nano-etched FS capillary * CE and MALDI methods * cell-surface adhesion from blood samples
Obor OECD: Analytical chemistry
Impakt faktor: 5.084, rok: 2020
Způsob publikování: Omezený přístup

The cells of S. aureus (methicillin susceptible or methicillin-resistant) and of P. aeruginosa were adhered onto nano-etched FS surface prepared by etching with SCW from 100 mL physiological saline solution, bovine serum, or human blood, and separated on-line by MEKC and off-line by MALDI-TOF MS. The limits of detection was from 180 to 1700 cells mL−1 at MEKC analysis and 4000 cells mL−1 at MALDI-TOF MS.
Trvalý link: http://hdl.handle.net/11104/0308397