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Hemocompatibility testing of blood-contacting implants in a flow loop model mimicking human blood flow
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SYSNO ASEP 0523815 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Hemocompatibility testing of blood-contacting implants in a flow loop model mimicking human blood flow Tvůrce(i) Link, A. (DE)
Cattaneo, G. (DE)
Brynda, Eduard (UMCH-V) RID
Riedel, Tomáš (UMCH-V) RID, ORCID
Kučerová, Johanka (UMCH-V)
Schlensak, C. (DE)
Wendel, H. P. (DE)
Krajewski, S. (DE)
Michel, T. (DE)Číslo článku e60610 Zdroj.dok. Jove-Journal of Visualized Experiments - ISSN 1940-087X
Roč. 2020, č. 157 (2020), s. 1-7Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova hemocompatibility ; flow loop model ; blood-contacting devices Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Biomaterials (as related to medical implants, devices, sensors) CEP LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA18-01163S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000523286100035 EID SCOPUS 85081670565 DOI 10.3791/60610 Anotace The growing use of medical devices (e.g., vascular grafts, stents, and cardiac catheters) for temporary or permanent purposes that remain in the body's circulatory system demands a reliable and multiparametric approach that evaluates the possible hematologic complications caused by these devices (i.e., activation and destruction of blood components). Comprehensive in vitro hemocompatibility testing of blood-contacting implants is the first step towards successful in vivo implementation. Therefore, extensive analysis according to the International Organization for Standardization 10993-4 (ISO 10993-4) is mandatory prior to clinical application. The presented flow loop describes a sensitive model to analyze the hemostatic performance of stents (in this case, neurovascular) and reveal adverse effects. The use of fresh human whole blood and gentle blood sampling are essential to avoid the preactivation of blood. The blood is perfused through a heparinized tubing containing the test specimen by using a peristaltic pump at a rate of 150 mL/min at 37 °C for 60 min. Before and after perfusion, hematologic markers (i.e., blood cell count, hemoglobin, hematocrit, and plasmatic markers) indicating the activation of leukocytes (polymorphonuclear [PMN]-elastase), platelets (β-thromboglobulin [β-TG]), the coagulation system (thombin-antithrombin III [TAT]), and the complement cascade (SC5b-9) are analyzed. In conclusion, we present an essential and reliable model for extensive hemocompatibility testing of stents and other blood-contacting devices prior to clinical application. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2021 Elektronická adresa https://www.jove.com/video/60610/hemocompatibility-testing-blood-contacting-implants-flow-loop-model
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