The biological effects of complete gasoline engine emissions exposure in a 3D human airway model (Mucilairtm) and in human bronchial epithelial cells (BEAS-2B)

Rössner ml., Pavel; Červená, Tereza; Vojtíšek-Lom, M.; Vrbová, Kristýna; Ambrož, Antonín; Nováková, Zuzana; Elzeinova, Fatima; Margaryan, Hasmik; Beránek, V.; Pechout, M.; Macoun, D.; Kléma, J.; Rössnerová, Andrea; Cigánek, M.; Topinka, Jan

doi:https://dx.doi.org/10.3390/ijms20225710

Počet záznamů: 1

The biological effects of complete gasoline engine emissions exposure in a 3D human airway model (Mucilairtm) and in human bronchial epithelial cells (BEAS-2B)

1.

SYSNO ASEP	0518531
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	The biological effects of complete gasoline engine emissions exposure in a 3D human airway model (Mucilairtm) and in human bronchial epithelial cells (BEAS-2B)
Tvůrce(i)	Rössner ml., Pavel (UEM-P)_{RID, ORCID} Červená, Tereza (UEM-P)_{ORCID, RID} Vojtíšek-Lom, M. (CZ) Vrbová, Kristýna (UEM-P) Ambrož, Antonín (UEM-P) Nováková, Zuzana (UEM-P) Elzeinova, Fatima (UEM-P) Margaryan, Hasmik (UEM-P) Beránek, V. (CZ) Pechout, M. (CZ) Macoun, D. (CZ) Kléma, J. (CZ) Rössnerová, Andrea (UEM-P)_RID Cigánek, M. (CZ) Topinka, Jan (UEM-P)_{RID, ORCID}
Číslo článku	5710
Zdroj.dok.	International Journal of Molecular Sciences. - : MDPI Roč. 20, č. 22 (2019)
Poč.str.	22 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	3D models ; cell monocultures ; complete engine emissions
Vědní obor RIV	EB - Genetika a molekulární biologie
Obor OECD	Toxicology
CEP	GA18-04719S GA ČR - Grantová agentura ČR
Způsob publikování	Open access
Institucionální podpora	UEM-P - RVO:68378041
UT WOS	000502786800192
EID SCOPUS	85075115173
DOI	10.3390/ijms20225710
Anotace	The biological effects induced by complete engine emissions in a 3D model of the human airway (MucilAir™) and in human bronchial epithelial cells (BEAS-2B) grown at the air–liquid interface were compared. The cells were exposed for one or five days to emissions generated by a Euro 5 direct injection spark ignition engine. The general condition of the cells was assessed by the measurement of transepithelial electrical resistance and mucin production. The cytotoxic effects were evaluated by adenylate kinase (AK) and lactate dehydrogenase (LDH) activity. Phosphorylation of histone H2AX was used to detect double-stranded DNA breaks. The expression of the selected 370 relevant genes was analyzed using next-generation sequencing. The exposure had minimal effects on integrity and AK leakage in both cell models. LDH activity and mucin production in BEAS-2B cells significantly increased after longer exposures, DNA breaks were also detected. The exposure affected CYP1A1 and HSPA5 expression in MucilAir™. There were no effects of this kind observed in BEAS-2B cells, in this system gene expression was rather affected by the time of treatment. The type of cell model was the most important factor modulating gene expression. In summary, the biological effects of complete emissions exposure were weak. In the specific conditions used in this study, the effects observed in BEAS-2B cells were induced by the exposure protocol rather than by emissions and thus this cell line seems to be less suitable for analyses of longer treatment than the 3D model.
Pracoviště	Ústav experimentální medicíny
Kontakt	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Rok sběru	2020
Elektronická adresa	https://www.mdpi.com/1422-0067/20/22/5710

Počet záznamů: 1