Počet záznamů: 1
Towards a quantitative assessment of inorganic carbon cycling in photosynthetic microorganisms
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SYSNO ASEP 0510250 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Towards a quantitative assessment of inorganic carbon cycling in photosynthetic microorganisms Tvůrce(i) Müller, S. (AT)
Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
Červený, Jan (UEK-B) RID, ORCID, SAIZdroj.dok. Engineering in Life Sciences. - : Wiley - ISSN 1618-0240
Roč. 19, č. 10 (2019), s. 1-13Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova carbonate chemistry ; computational modeling ; cyanobacteria ; futile cycles ; photosynthesis Vědní obor RIV EF - Botanika Obor OECD Plant sciences, botany CEP LO1415 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CzeCOS II - 90061 - Ústav výzkumu globální změny AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora UEK-B - RVO:86652079 UT WOS 000502733800001 DOI 10.1002/elsc.201900061 Anotace Photosynthetic organisms developed various strategies to mitigate high light stress. For instance, aquatic organisms are able to spend excessive energy by exchanging dissolved carbon dioxide (dCO2) and bicarbonate (HCO3-) with the environment. Simultaneous uptake and excretion of the two carbon species is referred to as inorganic carbon cycling (ICC). Often, ICC is indicated by displacements of the extracellular dCO2 signal from the equilibrium value after changing the light conditions. In this work, we additionally use (i) the extracellular pH signal, which requires non- or weakly-buffered medium, and (ii) a dynamic model of carbonate chemistry in the aquatic environment in order to detect and quantitatively describe ICC. Based on simulations and experiments in precisely controlled photobioreactors, we show that the magnitude of the observed dCO2 displacement crucially depends on extracellular pH level and buffer concentration. Moreover, we find that the dCO2 displacement can also be caused by simultaneous uptake of both dCO2 and HCO3- (no ICC). In a next step, the dynamic model of carbonate chemistry allows for a quantitative assessment of cellular dCO2 , HCO3- , and H+ exchange rates from the measured dCO2 and pH signals. Limitations of the method are discussed. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2020 Elektronická adresa https://onlinelibrary.wiley.com/doi/full/10.1002/elsc.201900061
Počet záznamů: 1