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HNK-1 in Morphological Study of Development of the Cardiac Conduction System in Selected Groups of Sauropsida
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SYSNO ASEP 0503883 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název HNK-1 in Morphological Study of Development of the Cardiac Conduction System in Selected Groups of Sauropsida Tvůrce(i) Kvasilová, A. (CZ)
Gregorovičová, Martina (FGU-C) RID, ORCID, SAI
Kundrát, M. (SK)
Sedmera, David (FGU-C) RID, ORCID, SAIZdroj.dok. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology. - : Wiley - ISSN 1932-8486
Roč. 302, č. 1 (2019), s. 69-82Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Leu 7 ; CD 57 ; immunohistochemistry ; heart ; reptile Vědní obor RIV FP - Ostatní lékařské obory Obor OECD Anatomy and morphology (plant science to be 1.6) CEP KJB6111301 GA AV ČR - Akademie věd GA16-02972S GA ČR - Grantová agentura ČR GA13-12412S GA ČR - Grantová agentura ČR LTC17023 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000459881400008 EID SCOPUS 85054860686 DOI 10.1002/ar.23925 Anotace Human natural killer (HNK)-1 antibody is an established marker of developing cardiac conduction system (CCS) in birds and mammals. In our search for the evolutionary origin of the CCS, we tested this antibody in a variety of sauropsid species (Crocodylus niloticus, Varanus indicus, Pogona vitticeps, Pantherophis guttatus, Eublepharis macularius, Gallus gallus, and Coturnix japonica). Hearts of different species were collected at various stages of embryonic development and studied to map immunoreactivity in cardiac tissues. We performed detection on alternating serial paraffin sections using immunohistochemistry for smooth muscle actin or sarcomeric actin as myocardial markers, and HNK-1 to visualize overall staining pattern and then positivity in specific myocyte populations. We observed HNK-1 expression of various intensity distributed in the extracellular matrix and mesenchymal cell surface of cardiac cushions in most of the examined hearts. Strong staining was found in the cardiac nerve fibers and ganglia in all species. The myocardium of the sinus venosus and the atrioventricular canal exhibited transitory patterns of expression. In the Pogona and Crocodylus hearts, as well as in the Gallus and Coturnix ones, additional expression was detected in a subset of myocytes of the (inter)ventricular septum. These results support the use of HNK-1 as a conserved marker of the CCS and suggest that there is a rudimentary CCS present in developing reptilian hearts. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa https://doi.org/10.1002/ar.23925
Počet záznamů: 1