Počet záznamů: 1
Characterization of CD46 and β1 integrin dynamics during sperm acrosome reaction
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SYSNO ASEP 0463058 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization of CD46 and β1 integrin dynamics during sperm acrosome reaction Tvůrce(i) Frolíková, Michaela (BTO-N) ORCID
Šebková, Nataša (BTO-N)
Děd, Lukáš (BTO-N) RID
Dvořáková-Hortová, Kateřina (BTO-N)Celkový počet autorů 4 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 6, Sep 26 (2016), s. 1-15Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova CD46 ; β1 integrin ; acrosome reaction ; actin ; SIM ; latrunculin A Vědní obor RIV EC - Imunologie CEP GA14-05547S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 UT WOS 000384476400001 EID SCOPUS 84988878394 DOI 10.1038/srep33714 Anotace The acrosome reaction (AR) is a process of membrane fusion and lytic enzyme release, which enables sperm to penetrate the egg surroundings. It is widely recognized that specific sperm proteins form an active network prior to fertilization, and their dynamic relocation is crucial for the sperm-egg fusion. The unique presence of the membrane cofactor protein CD46 in the sperm acrosomal membrane was shown, however, its behaviour and connection with other sperm proteins has not been explored further. Using super resolution microscopy, we demonstrated a dynamic CD46 reorganisation over the sperm head during the AR, and its interaction with transmembrane protein integrins, which was confirmed by proximity ligation assay. Furthermore, we propose their joint involvement in actin network rearrangement. Moreover, CD46 and β1 integrins with subunit α3, but not α6, are localized into the apical acrosome and are expected to be involved in signal transduction pathways directing the acrosome stability and essential protein network rearrangements prior to gamete fusion. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2017
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