Počet záznamů: 1  

Characterization of CD46 and β1 integrin dynamics during sperm acrosome reaction

  1. 1.
    SYSNO ASEP0463058
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevCharacterization of CD46 and β1 integrin dynamics during sperm acrosome reaction
    Tvůrce(i) Frolíková, Michaela (BTO-N) ORCID
    Šebková, Nataša (BTO-N)
    Děd, Lukáš (BTO-N) RID
    Dvořáková-Hortová, Kateřina (BTO-N)
    Celkový počet autorů4
    Zdroj.dok.Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
    Roč. 6, Sep 26 (2016), s. 1-15
    Poč.str.15 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovaCD46 ; β1 integrin ; acrosome reaction ; actin ; SIM ; latrunculin A
    Vědní obor RIVEC - Imunologie
    CEPGA14-05547S GA ČR - Grantová agentura ČR
    ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaBTO-N - RVO:86652036
    UT WOS000384476400001
    EID SCOPUS84988878394
    DOI10.1038/srep33714
    AnotaceThe acrosome reaction (AR) is a process of membrane fusion and lytic enzyme release, which enables sperm to penetrate the egg surroundings. It is widely recognized that specific sperm proteins form an active network prior to fertilization, and their dynamic relocation is crucial for the sperm-egg fusion. The unique presence of the membrane cofactor protein CD46 in the sperm acrosomal membrane was shown, however, its behaviour and connection with other sperm proteins has not been explored further. Using super resolution microscopy, we demonstrated a dynamic CD46 reorganisation over the sperm head during the AR, and its interaction with transmembrane protein integrins, which was confirmed by proximity ligation assay. Furthermore, we propose their joint involvement in actin network rearrangement. Moreover, CD46 and β1 integrins with subunit α3, but not α6, are localized into the apical acrosome and are expected to be involved in signal transduction pathways directing the acrosome stability and essential protein network rearrangements prior to gamete fusion.
    PracovištěBiotechnologický ústav
    KontaktMonika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
    Rok sběru2017
Počet záznamů: 1  

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