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Towards Keratan Sulfate - Chemoenzymatic Cascade Synthesis of Sulfated N-Acetyllactosamine (LacNAc) Glycan Oligomers
- 1.0460034 - MBÚ 2017 RIV DE eng J - Článek v odborném periodiku
Lange, B. - Šimonová, Anna - Fischoeder, T. - Pelantová, Helena - Křen, Vladimír - Elling, L.
Towards Keratan Sulfate - Chemoenzymatic Cascade Synthesis of Sulfated N-Acetyllactosamine (LacNAc) Glycan Oligomers.
Advanced Synthesis & Catalysis. Roč. 358, č. 4 (2016), s. 584-596. ISSN 1615-4150. E-ISSN 1615-4169
Grant CEP: GA MŠMT(CZ) LD13042; GA ČR GC15-02578J
Institucionální podpora: RVO:61388971
Klíčová slova: biocatalysis * carbohydrates * glycoconjugates
Kód oboru RIV: CE - Biochemie
Impakt faktor: 5.646, rok: 2016
We report on a novel chemoenzymatic cascade for the synthesis of sulfated N-acetyllactosamine [(3Gal1,4GlcNAc1,)(n), LacNAc] oligomer structures. Starting from a linker modified GlcNAc substrate di- and trisaccharides were first synthesized by sequential use of human 4-galactosyltransferase-1 (4GalT-1) and 3-N-acetylglucosaminyltransferase from Helicobacter pylori (3GlcNAcT). Subsequent regioselective chemical sulfation rendered the C-6 mono-, di-, and tri-O-sulfated products in good yields. Further enzymatic elongation by 4GalT-1 and 3GlcNAcT in a sequential mode yielded 6-O-sulfated LacNAc oligomers up to hexasaccharide length with variable degrees of sulfation. These carbohydrate structures mimic the sulfation pattern found in keratan sulfate and are potential ligands for different classes of glycan binding proteins.
Trvalý link: http://hdl.handle.net/11104/0260184
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