Počet záznamů: 1
Double-strand break repair and colorectal cancer: gene variants within 3' UTRs and microRNAs binding as modulators of cancer risk and clinical outcome.
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SYSNO ASEP 0459496 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Double-strand break repair and colorectal cancer: gene variants within 3' UTRs and microRNAs binding as modulators of cancer risk and clinical outcome. Tvůrce(i) Naccarati, Alessio (UEM-P)
Rosa, F. (IT)
Vymetálková, Veronika (UEM-P) RID
Barone, E. (IT)
Jirásková, Kateřina (UEM-P)
Gaetano, C. (IT)
Novotný, J. (CZ)
Levý, M. (CZ)
Vodičková, Ludmila (UEM-P) RID
Gemignani, F. (IT)
Buchler, T. (CZ)
Landi, S. (IT)
Vodička, Pavel (UEM-P) RID
Pardini, B. (IT)Zdroj.dok. OncoTarget. - : Impact Journals LLC - ISSN 1949-2553
Roč. 7, č. 17 (2016), s. 23156-23169Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova 3'UTR polymorphisms ; colorectal cancer risk and clinical outcomes ; double-strand break repair (DSBR) genes Vědní obor RIV EB - Genetika a molekulární biologie CEP NV15-26535A GA MZd - Ministerstvo zdravotnictví GAP304/12/1585 GA ČR - Grantová agentura ČR GA15-14789S GA ČR - Grantová agentura ČR Institucionální podpora UEM-P - RVO:68378041 UT WOS 000377706200015 EID SCOPUS 84966549132 DOI 10.18632/oncotarget.6804 Anotace Genetic variations in 3' untranslated regions of target genes may affect microRNA binding, resulting in differential protein expression. microRNAs regulate DNA repair, and single-nucleotide polymorphisms in miRNA binding sites (miRSNPs) may account for interindividual differences in the DNA repair capacity. Our hypothesis is that miRSNPs in relevant DNA repair genes may ultimately affect cancer susceptibility and impact prognosis. In the present study, we analysed the association of polymorphisms in predicted microRNA target sites of double-strand breaks (DSBs) repair genes with colorectal cancer (CRC) risk and clinical outcome. Twenty-one miRSNPs in non-homologous end-joining and homologous recombination pathways were assessed in 1111 cases and 1469 controls. The variant CC genotype of rs2155209 in MRE11A was strongly associated with decreased cancer risk when compared with the other genotypes (OR 0.54, 95% CI 0.38-0.76, p = 0.0004). A reduced expression of the reporter gene was observed for the C allele of this polymorphism by in vitro assay, suggesting a more efficient interaction with potentially binding miRNAs. In colon cancer patients, the rs2155209 CC genotype was associated with shorter survival while the TT genotype of RAD52 rs11226 with longer survival when both compared with their respective more frequent genotypes (HR 1.63, 95% CI 1.06-2.51, p = 0.03 HR 0.60, 95% CI 0.41-0.89, p = 0.01, respectively). miRSNPs in DSB repair genes involved in the maintenance of genomic stability may have a role on CRC susceptibility and clinical outcome. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2017
Počet záznamů: 1