Počet záznamů: 1
Phylogeny and morphological variability of trypanosomes from African pelomedusid turtles with redescription of Trypanosoma mocambicum Pienaar, 1962
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SYSNO ASEP 0454867 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Phylogeny and morphological variability of trypanosomes from African pelomedusid turtles with redescription of Trypanosoma mocambicum Pienaar, 1962 Tvůrce(i) Dvořáková, N. (CZ)
Čepička, I. (CZ)
Qablan, M. A. (CZ)
Gibson, W. (GB)
Blažek, Radim (UBO-W) RID, ORCID, SAI
Široký, P. (CZ)Celkový počet autorů 6 Zdroj.dok. Protist. - : Elsevier - ISSN 1434-4610
Roč. 166, č. 6 (2015), s. 599-608Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Trypanosoma ; turtle ; Pelusios ; polymorphism ; phylogeny ; SSU rRNA gene Vědní obor RIV EG - Zoologie Institucionální podpora UBO-W - RVO:68081766 UT WOS 000366806600001 EID SCOPUS 84947436474 DOI 10.1016/j.protis.2015.10.002 Anotace Little is known about host specificity, genetic diversity and phylogenetic relationships of African turtle trypanosomes. Using PCR targeting the SSU rRNA gene, we detected trypanosomes in 24 of 134 (17.9%) wild caught African pelomedusid turtles: Pelusios upembae (n = 14), P. bechuanicus (n = 1), P. rhodesianus (n = 3) and P. subniger (n = 6). Mixed infection of Trypanosoma species was confirmed by PCR in three specimens of P. upembae, and in one specimen each of P. bechuanicus, P. rhodesianus, and P. subniger. Microscopic examination of stained blood smears revealed two distinct forms (broad and slender) of trypomastigotes. The broad form coincided in morphology with T. mocambicum Pienaar, 1962. Accordingly, we have designated this form as the neotype of T. mocambicum. In phylogenetic analysis of the SSU rRNA gene, all the new turtle trypanosome sequences grouped in a single clade within the strongly supported "aquatic" clade of Trypanosoma species. The turtle trypanosome clade was further subdivided into two subclades, which did not correlate with host turtle species or trypanosome morphology. This study provides the first sequence data of Trypanosoma species isolated from freshwater turtles from tropical Africa and extends knowledge on diversity of trypanosomes in the Afrotropical zoogeographical realm. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2016
Počet záznamů: 1