Počet záznamů: 1
Quantification of potassium levels in cells treated with Bordetella adenylate cyclase toxin
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SYSNO ASEP 0428410 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Quantification of potassium levels in cells treated with Bordetella adenylate cyclase toxin Tvůrce(i) Wald, Tomáš (MBU-M) RID
Petry-Podgórska, Inga (UIACH-O)
Fišer, Radovan (MBU-M) RID, ORCID
Matoušek, Tomáš (UIACH-O) RID, ORCID
Dědina, Jiří (UIACH-O) RID, ORCID
Osička, Radim (MBU-M) RID, ORCID
Šebo, Peter (MBU-M) RID, ORCID
Mašín, Jiří (MBU-M) RID, ORCIDZdroj.dok. Analytical Biochemistry. - : Elsevier - ISSN 0003-2697
Roč. 450, APR 2014 (2014), s. 57-62Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Potassium ; Adenylate cyclase toxin ; RTX Vědní obor RIV CE - Biochemie CEP GAP302/11/0580 GA ČR - Grantová agentura ČR GA13-14547S GA ČR - Grantová agentura ČR GAP302/12/0460 GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 ; UIACH-O - RVO:68081715 UT WOS 000333500900011 DOI 10.1016/j.ab.2013.10.039 Anotace The aim of this study was to compare two methods for quantification of changes in intracellular potassium concentration (decrease from similar to 140 to similar to 20 mM) due to the action of a pore-forming toxin, the adenylate cyclase toxin (CyaA) from the pathogenic bacterium Bordetella pertussis. CyaA was incubated with stably transfected K1 Chinese hamster ovary cells expressing the toxin receptor CD11b/CD18 and the decrease in potassium concentration in the cells was followed by inductively coupled plasma mass spectrometry (ICP-MS). It is shown that this method is superior in terms of sensitivity, accuracy, and temporal resolution over the method employing the potassium-binding benzofuran isophthalate-acetoxymethyl ester fluorescent indicator. The ICP-MS procedure was found to be a reliable and straightforward analytical approach enabling kinetic studies of CyaA action at physiologically relevant toxin concentrations (<1000 ng/ml) in biological microsamples Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2015
Počet záznamů: 1