Počet záznamů: 1
The role of desaturases in the biosynthesis of marking pheromones in bumblebee males
- 1.
SYSNO ASEP 0395109 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The role of desaturases in the biosynthesis of marking pheromones in bumblebee males Tvůrce(i) Buček, Aleš (UOCHB-X) RID
Vogel, H. (DE)
Matoušková, Petra (UOCHB-X)
Prchalová, Darina (UOCHB-X)
Žáček, Petr (UOCHB-X) RID
Vrkoslav, Vladimír (UOCHB-X) RID, ORCID
Šebesta, Petr (UOCHB-X)
Svatoš, Aleš (UOCHB-X) ORCID, RID
Jahn, Ullrich (UOCHB-X) ORCID
Valterová, Irena (UOCHB-X) RID, ORCID
Pichová, Iva (UOCHB-X) RID, ORCIDCelkový počet autorů 11 Zdroj.dok. Insect Biochemistry and Molecular Biology. - : Elsevier - ISSN 0965-1748
Roč. 43, č. 8 (2013), s. 724-731Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova fatty acid desaturase ; bumblebee ; Hymenoptera ; pheromone ; RNA-seq ; functional expression Vědní obor RIV CE - Biochemie CEP GA203/09/1446 GA ČR - Grantová agentura ČR Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000322561700011 EID SCOPUS 84879484391 DOI 10.1016/j.ibmb.2013.05.003 Anotace Bumblebee males (Hymenoptera) produce species-specific labial gland secretions called marking pheromones (MPs). MPs generally consist of terpenoids and fatty-acid-derived aliphatic compounds with various chain lengths predominantly containing one or no double bonds. The unsaturated fatty-acid-derived MP components were hypothesized to be produced by fatty acid desaturases (FADs) that exhibit diverse substrate specificities. To address this hypothesis, we isolated and functionally characterized FADs from three bumblebee species: Bombus lucorum, Bombus terrestris, and Bombus lapidarius. By employing RNA sequencing of the male labial glands and fat bodies of B. lucorum and B. terrestris, we identified five paralogous FAD-like sequences but only two FAD lineages were abundant and differentially expressed in the labial glands. We found that abundant FAD lineages were also expressed in the labial gland and fat body of Bombus lapidarius. Functional characterization of FADs in a yeast expression system confirmed that Delta 4-FADs exhibited a unique Delta 4-desaturase activity exclusively on 14-carbon fatty acyls and Delta 9-FADs displayed Delta 9-desaturase activity on 14- to 18-carbon fatty acyls. These results indicate that Delta 9-FADs are involved in the biosynthesis of major unsaturated components of MPs in B. lucorum and B. lapidarius despite the diverse MP composition of these bumblebee species. The contribution of lipases, acyltransferases, esterases, and fatty acid reductases to production of the species-specific MP composition is also discussed in light of the transcriptomic data obtained in this study. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2014
Počet záznamů: 1