Počet záznamů: 1
The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography
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SYSNO ASEP 0364545 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography Tvůrce(i) Czauderna, M. (PL)
Kowalczyk, J. (PL)
Marounek, Milan (UZFG-Y) RIDCelkový počet autorů 3 Zdroj.dok. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 879, č. 23 (2011), s. 2251-2258Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Malondialdehyde ; 2,4-Dinitrophenylhydrazine ; High performance liquid chromatography Vědní obor RIV CB - Analytická chemie, separace CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000293722800012 DOI 10.1016/j.jchromb.2011.06.008 Anotace A method for the determination of malondialdehyde (MDA) concentrations in specimens of animal tissues and feed has been developed using high performance liquid chromatography. The MDA concentration in acidified urine samples was determined after its conversion with 2,4-dinitrophenylhydrazine (DNPH) to a hydrazone (MDA-DNPH). Samples of blood plasma, muscle, liver and feed were prepared by saponification followed by derivatisation with DNPH to MDA-DNPH. The MDA concentration in chicken and hen feed samples was analysed after saponification and derivatisation followed by extractions with hexane. The free MDA in plasma samples was determined after deproteinization followed by derivatisation of MDA with DNPH. The chromatographic separation of MDA-DNPH samples was conducted using Phenomenex C(18)-columns (Synergi 2.5 mu m, Hydro-RP, 100 angstrom, the length of 100 mm) with an inner diameter of 2 or 3 mm. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2012
Počet záznamů: 1