Počet záznamů: 1
A novel SATB1 protein isoform with different biophysical properties
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SYSNO ASEP 0575263 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A novel SATB1 protein isoform with different biophysical properties Tvůrce(i) Zelenka, T. (GR)
Papamatheakis, D. (GR)
Tzerpos, P. (GR)
Panagopoulos, G. (GR)
Tsolis, K. C. (GR)
Papadakis, V. M. (GR)
Mariatos Metaxas, D. (GR)
Papadogkonas, G. (GR)
Mores, E. (GR)
Kapsetaki, M. (GR)
Papamatheakis, J. (GR)
Staněk, David (UMG-J) RID
Spilianakis, C. (GR)Celkový počet autorů 13 Číslo článku 1242481 Zdroj.dok. Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 11, Aug (2023)Poč.str. 22 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova satb1 ; T cells ; chromatin organization ; prion ; phase separation Obor OECD Cell biology CEP LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF18_046/0016045 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 001054274200001 EID SCOPUS 85169128313 DOI 10.3389/fcell.2023.1242481 Anotace Intra-thymic T cell development is coordinated by the regulatory actions of SATB1 genome organizer. In this report, we show that SATB1 is involved in the regulation of transcription and splicing, both of which displayed deregulation in Satb1 knockout murine thymocytes. More importantly, we characterized a novel SATB1 protein isoform and described its distinct biophysical behavior, implicating potential functional differences compared to the commonly studied isoform. SATB1 utilized its prion-like domains to transition through liquid-like states to aggregated structures. This behavior was dependent on protein concentration as well as phosphorylation and interaction with nuclear RNA. Notably, the long SATB1 isoform was more prone to aggregate following phase separation. Thus, the tight regulation of SATB1 isoforms expression levels alongside with protein post-translational modifications, are imperative for SATB1's mode of action in T cell development. Our data indicate that deregulation of these processes may also be linked to disorders such as cancer. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2024 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fcell.2023.1242481/full
Počet záznamů: 1