Počet záznamů: 1
Development of novel fluorescent tags for glycosylation analysis by CE/LIF and CE-MS
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SYSNO ASEP 0573238 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Development of novel fluorescent tags for glycosylation analysis by CE/LIF and CE-MS Tvůrce(i) Lavická, Jana (UIACH-O) ORCID, RID
Smolková, Denisa (UIACH-O)
Čmelík, Richard (UIACH-O) RID
Duša, Filip (UIACH-O) RID, ORCIDCelkový počet autorů 4 Zdroj.dok. MSB 2023. -, 2023
S. 1-1Poč.str. 1 s. Forma vydání Online - E Akce MSB 2023. International Symposium on Microscale Separations and Bioanalysis /39./ Datum konání 21.05.2023 - 24.05.2023 Místo konání Florida State University, Tallahassee Země US - Spojené státy americké Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova CE/LIF ; CE-MS ; glycosylation analysis Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA22-00236S GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 Anotace In this work, we present design, synthesis, and application of new fluorescent tags providing rapid labeling kinetics, high fluorescence quantum yields, and significantly enhanced detectability by MS. The new fluorescent tags based on pyrene, coumarin and/or BODIPY derivatives with various substituents and modifiable linkers were synthetized and the desired modification allowed to tune the fluorescence and MS properties (preferred wavelength and quantum yield, charge). A detailed comparison of the mechanisms of labeling, reductive amination and hydrazone formation chemistries, showed that the labeling yield obtained by hydrazone formation chemistry is almost one order of magnitude higher compared to the reductive amination approach. Furthermore, the undesired formation of multiple products of N-linked glycan labeling by reductive amination was detected when positively charged labels such as aliphatic/aromatic amines containing a quaternary ammonium group were attached. Labeling with different synthetized tags as well as their comparison with the commercially available labels will be demonstrated by analysis maltooligosaccharides and N-linked glycans by CE/LIF and CE-MS. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2024
Počet záznamů: 1