Počet záznamů: 1
Blood-feeding adaptations and virome assessment of the poultry red mite Dermanyssus gallinae guided by RNA-seq
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SYSNO ASEP 0572373 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Blood-feeding adaptations and virome assessment of the poultry red mite Dermanyssus gallinae guided by RNA-seq Tvůrce(i) Ribeiro, J.M. (US)
Hartmann, David (BC-A) ORCID, RID
Bartošová-Sojková, Pavla (BC-A) RID, ORCID
Debat, H. (AR)
Moos, Martin (BC-A) RID, ORCID
Šimek, Petr (BC-A) RID, ORCID
Fara, J. (CZ)
Palus, Martin (BC-A) RID, ORCID
Kučera, Matěj (BC-A)
Hajdušek, Ondřej (BC-A) RID, ORCID
Sojka, Daniel (BC-A) RID, ORCID
Kopáček, Petr (BC-A) RID, ORCID
Perner, Jan (BC-A) RID, ORCIDCelkový počet autorů 13 Číslo článku 517 Zdroj.dok. Communications Biology. - : Nature Publishing Group
Roč. 6, č. 1 (2023)Poč.str. 15 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Blood-feeding ; Dermanyssus gallinae ; RNA-seq Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP TJ02000107 GA TA ČR - Technologická agentura ČR GM22-18424M GA ČR - Grantová agentura ČR GA20-05736S GA ČR - Grantová agentura ČR GA21-08826S GA ČR - Grantová agentura ČR EF16_019/0000759 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora BC-A - RVO:60077344 UT WOS 000986440900004 EID SCOPUS 85159740952 DOI 10.1038/s42003-023-04907-x Anotace Dermanyssus gallinae is a blood-feeding mite that parasitises wild birds and farmed poultry. Its remarkably swift processing of blood, together with the capacity to blood-feed during most developmental stages, makes this mite a highly debilitating pest. To identify specific adaptations to digestion of a haemoglobin-rich diet, we constructed and compared transcriptomes from starved and blood-fed stages of the parasite and identified midgut-enriched transcripts. We noted that midgut transcripts encoding cysteine proteases were upregulated with a blood meal. Mapping the full proteolytic apparatus, we noted a reduction in the suite of cysteine proteases, missing homologues for Cathepsin B and C. We have further identified and phylogenetically analysed three distinct transcripts encoding vitellogenins that facilitate the reproductive capacity of the mites. We also fully mapped transcripts for haem biosynthesis and the ferritin-based system of iron storage and inter-tissue trafficking. Additionally, we identified transcripts encoding proteins implicated in immune signalling (Toll and IMD pathways) and activity (defensins and thioester-containing proteins), RNAi, and ion channelling (with targets for commercial acaricides such as Fluralaner, Fipronil, and Ivermectin). Viral sequences were filtered from the Illumina reads and we described, in part, the RNA-virome of D. gallinae with identification of a novel virus, Red mite quaranjavirus 1. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2024 Elektronická adresa https://www.nature.com/articles/s42003-023-04907-x
Počet záznamů: 1