Počet záznamů: 1
The Ixodes ricinus salivary gland proteome during feeding and B. Afzelii infection: New avenues for an anti-tick vaccine
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SYSNO ASEP 0571515 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Ixodes ricinus salivary gland proteome during feeding and B. Afzelii infection: New avenues for an anti-tick vaccine Tvůrce(i) Klouwens, M.J. (NL)
Trentelman, J.J.A. (NL)
Barriales, D. (ES)
Ersoz, J. (NL)
Azkargorta, M. (ES)
Elortza, F. (ES)
Šíma, Radek (BC-A) RID, ORCID
Hajdušek, Ondřej (BC-A) RID, ORCID
Cortazar, J.T. (ES)
Corcuera, I. E. (ES)
Colstrup, E. (NL)
Nayak, A. (NL)
Ruiz, I.M. (ES)
Rodriguez, H. (ES)
Nijhof, A.M. (DE)
Anguita, J. (ES)
Hovius, J.W.R. (NL)Celkový počet autorů 17 Zdroj.dok. Vaccine. - : Elsevier - ISSN 0264-410X
Roč. 41, č. 12 (2023), s. 1951-1960Poč.str. 10 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Borrelia afzelii ; Proteomics ; Ixodes ricinus ; Salivary glands ; Feeding ; Antitick vaccines Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP EF16_019/0000759 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NU20-05-00396 GA MZd - Ministerstvo zdravotnictví GA22-30920S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000965928900001 EID SCOPUS 85148363669 DOI 10.1016/j.vaccine.2023.02.003 Anotace Introduction: Borrelia burgdorferi sensu lato, the causative agents of Lyme borreliosis, are transmitted by Ixodes ticks. Tick saliva proteins are instrumental for survival of both the vector and spirochete and have been investigated as targets for vaccine targeting the vector. In Europe, the main vector for Lyme borre-liosis is Ixodes ricinus, which predominantly transmits Borrelia afzelii. We here investigated the differen-tial production of I. ricinus tick saliva proteins in response to feeding and B. afzelii infection.Method: Label-free Quantitative Proteomics and Progenesis QI software was used to identify, compare, and select tick salivary gland proteins differentially produced during tick feeding and in response to B. afzelii infection. Tick saliva proteins were selected for validation, recombinantly expressed and used in both mouse and guinea pig vaccination and tick-challenge studies.Results: We identified 870 I. ricinus proteins from which 68 were overrepresented upon 24-hours of feed-ing and B. afzelii infection. Selected tick proteins were successfully validated by confirming their expres-sion at the RNA and native protein level in independent tick pools. When used in a recombinant vaccine formulation, these tick proteins significantly reduced the post-engorgement weights of I. ricinus nymphs in two experimental animal models. Despite the reduced ability of ticks to feed on vaccinated animals, we observed efficient transmission of B. afzelii to the murine host.Conclusion: Using quantitative proteomics, we identified differential protein production in I. ricinus sali-vary glands in response to B. afzelii infection and different feeding conditions. These results provide novel insights into the process of I. ricinus feeding and B. afzelii transmission and revealed novel candidates for an anti-tick vaccine.(c) 2023 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2024 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0264410X23001299?via%3Dihub
Počet záznamů: 1