Počet záznamů: 1
Meis2 controls skeletal formation in the hyoid region
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SYSNO ASEP 0568810 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Meis2 controls skeletal formation in the hyoid region Tvůrce(i) Fábik, Jaroslav (UEM-P)
Psutková, Viktorie (UEM-P)
Machoň, Ondřej (UEM-P)Číslo článku 951063 Zdroj.dok. Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 10, sep. (2022)Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Meis2 ; cartilage ; hyoid bone ; Hand2 ; PBX1 ; mesenchymal condensation Obor OECD Developmental biology CEP GA22-10660S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 000868663800001 EID SCOPUS 85139797403 DOI 10.3389/fcell.2022.951063 Anotace A vertebrate skull is composed of many skeletal elements which display enormous diversity of shapes. Cranial bone formation embodies a multitude of processes, i.e., epithelial-mesenchymal induction, mesenchymal condensation, and endochondral or intramembranous ossification. Molecular pathways determining complex architecture and growth of the cranial skeleton during embryogenesis are poorly understood. Here, we present a model of the hyoid apparatus development in Wnt1-Cre2-induced Meis2 conditional knock-out (cKO) mice. Meis2 cKO embryos develop an aberrant hyoid apparatus-a complete skeletal chain from the base of the neurocranium to lesser horns of the hyoid, resembling extreme human pathologies of the hyoid-larynx region. We examined key stages of hyoid skeletogenesis to obtain a complex image of the hyoid apparatus formation. Lack of Meis2 resulted in ectopic loci of mesenchymal condensations, ectopic cartilage and bone formation, disinhibition of skeletogenesis, and elevated proliferation of cartilage precursors. We presume that all these mechanisms contribute to formation of the aberrant skeletal chain in the hyoid region. Moreover, Meis2 cKO embryos exhibit severely reduced expression of PBX1 and HAND2 in the hyoid region. Altogether, MEIS2 in conjunction with PBX1 and HAND2 affects mesenchymal condensation, specification and proliferation of cartilage precursors to ensure development of the anatomically correct hyoid apparatus. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2023 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fcell.2022.951063/full
Počet záznamů: 1