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Is Renal beta-Adrenergic-WNK4-NCC Pathway Important in Salt Hypertension of Dahl Rats?
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SYSNO ASEP 0565778 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Is Renal beta-Adrenergic-WNK4-NCC Pathway Important in Salt Hypertension of Dahl Rats? Tvůrce(i) Zicha, Josef (FGU-C) RID, ORCID, SAI
Hojná, Silvie (FGU-C) RID, ORCID
Vaňourková, Z. (CZ)
Kopkan, L. (CZ)
Vaněčková, Ivana (FGU-C) RID, ORCIDZdroj.dok. Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 68, č. 6 (2019), s. 873-882Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova sympathetic nervous system ; beta-adrenergic blockade ; renal sodium excretion ; NCC cotransporter ; hydrochlorothiazide ; serine/threonine-protein kinase WNK4 Obor OECD Physiology (including cytology) CEP NV15-25396A GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000505181300001 EID SCOPUS 85077404400 DOI 10.33549/physiolres.934334 Anotace In 2011 Fujita and coworkers proposed that beta-adrenergic stimulation causes decreased serine/threonine-protein kinase WNK4 transcription leading to the activation of Na-CI cotransporter (NCC) which participates in salt sensitivity and salt hypertension development in rodents. The aim of our study was to investigate whether the above hypothesis is also valid for salt hypertension of Dahl rats, which are characterized by high sympathetic tone and abnormal renal sodium handling. Male 8-week-old salt-sensitive (SS/Jr) and salt-resistant (SR/Jr) Dahl rats were fed either low-salt diet (LS, 0.4 % NaCI) or high-salt diet (HS, 4 % NaCI) for 6 weeks. Half of the animals on either diet were chronically treated with non-selective 8-blocker propranolol (100 mg/kg/day). At the end of the experiment diuresis and sodium excretion were measured prior and after hydrochlorothiazide injection (HCTZ, 10 mg/kg i.p.). Furthermore, blood pressure (BP), heart rate (HR), sympathetic (pentolinium 5 mg/kg i.v.) and NO-dependent (L-NAME 30 mg/kg i.v.) BP components were determined. Chronic HS diet feeding increased BP through sympathoexcitation in SS/Jr but not in SR/Jr rats. Concomitant propranolol treatment did not lower BP in either experimental group. Under the conditions of low salt intake HCTZ increased diuresis, natriuresis and fractional sodium excretion in SR/Jr but not in SS/Jr rats. HS diet feeding attenuated renal response to HCT in SR/Jr rats, whereas no HCTZ effect was observed in SS/Jr rats fed HS diet. Propranolol treatment did not modify diuresis or natriuresis in any experimental group. In conclusions, our present data do not support the idea on the essential importance of renal beta-adrenergic-WNK4-NCC pathway in pathogenesis and/or maintenance of salt hypertension in Dahl rats. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2023 Elektronická adresa http://www.biomed.cas.cz/physiolres/pdf/2019/68_873.pdf
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