Počet záznamů: 1
Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification
- 1.
SYSNO ASEP 0559191 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification Tvůrce(i) Petitpre, Ch. (SE)
Faure, L. (AT)
Uhl, P. (SE)
Fontanet, P. (SE)
Filova, Iva (BTO-N) ORCID
Pavlínková, Gabriela (BTO-N) RID, ORCID
Adameyko, I. (AT)
Hadjab, S. (SE)
Lallemend, F. (SE)Celkový počet autorů 9 Číslo článku 3878 Zdroj.dok. Nature Communications. - : Nature Publishing Group
Roč. 13, č. 1 (2022)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova SPIRAL GANGLION NEURONS ; HAIR-CELLS ; DEVELOPMENTAL REGULATION Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology CEP GA20-06927S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000821901200027 EID SCOPUS 85133477774 DOI 10.1038/s41467-022-31580-1 Anotace Different types of spiral ganglion neurons (SGNs) are essential for auditory perception by transmitting complex auditory information from hair cells (HCs) to the brain. Here, we use deep, single cell transcriptomics to study the molecular mechanisms that govern their identity and organization in mice. We identify a core set of temporally patterned genes and gene regulatory networks that may contribute to the diversification of SGNs through sequential binary decisions and demonstrate a role for NEUROD1 in driving specification of a I-c-SGN phenotype. We also find that each trajectory of the decision tree is defined by initial co-expression of alternative subtype molecular controls followed by gradual shifts toward cell fate resolution. Finally, analysis of both developing SGN and HC types reveals cell-cell signaling potentially playing a role in the differentiation of SGNs. Our results indicate that SGN identities are drafted prior to birth and reveal molecular principles that shape their differentiation and will facilitate studies of their development, physiology, and dysfunction. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2023 Elektronická adresa https://www.nature.com/articles/s41467-022-31580-1
Počet záznamů: 1