Počet záznamů: 1
New insights into mechanisms of copper nanoparticle toxicity in freshwater algae Chlamydomonas reinhardtii: Effects on the pathways of secondary metabolites
- 1.
SYSNO ASEP 0551735 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název New insights into mechanisms of copper nanoparticle toxicity in freshwater algae Chlamydomonas reinhardtii: Effects on the pathways of secondary metabolites Tvůrce(i) Janová, A. (CZ)
Koláčková, M. (CZ)
Bytesnikova, Z. (CZ)
Cápal, Petr (UEB-Q) RID, ORCID
Chaloupsky, P. (CZ)
Svec, P. (SK)
Ridoskova, A. (CZ)
Cernei, N. (CZ)
Klejdus, B. (CZ)
Richtera, L. (CZ)
Adam, V. (CZ)
Húska, D. (CZ)Rok vydání 2021 Číslo článku 102476 Zdroj.dok. Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 60, DEC (2021)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova oxide nanoparticles ; cuo nanoparticles ; green-alga ; chlorella-pyrenoidosa ; scenedesmus-obliquus ; zno nanoparticles ; elevated levels ; microalgae ; exposure ; stress ; Copper nanoparticles ; Chlamydomonas ; Toxicity ; Penetration ; Secondary metabolites ; Nanoparticle stability Obor OECD Nano-materials (production and properties) Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000696319800001 EID SCOPUS 85114180734 DOI 10.1016/j.algal.2021.102476 Anotace The effects of copper nanoparticles (Cu-NPs), including their stability in the medium, were studied with the green unicellular algae Chlamydomonas reinhardtii (CC-125). Cu-NPs were synthesized and characterized. Cu-NP particles were uniform, regular, and largely spherical, and they had smooth surfaces, the average size was estimated to be 137.4 +/- 2.1 nm. Chlamydomonas cells were cultivated for 96 h under controlled conditions in the presence of Cu-NPs, according to OECD guidelines, and then subjected to toxicological bioassays. Based on scanning electron microscopy (SEM) observations, the effects of Cu-NPs resulted in part from the dissolution of nanoparticles (NPs) and the action of copper itself, which shows the importance of studying NP stability in the testing environment. In this assay, deleterious effects were enhanced by increasing Cu-NP concentrations (5, 10, 25, 50, and 100 mg/L). Concentrations higher than 25 mg/L exhibited extreme toxicity. We confirmed the known toxic effects of metal NPs, namely, growth inhibition, reduction of chlorophyll levels in cells, cell penetration and increased ROS production. Attention was also paid to select underexplored metabolites, which were studied with a LC-MS/MS system. Treatments caused changes in metabolites profiles, and levels of p-hydroxybenzaldehyde and protocatechuic acid were especially enhanced, suggesting their positive roles in the antioxidant defence response. Furthermore, a repeatable increase in suberic acid levels was observed for various stress conditions tested, and we expect that this was the result of lipid peroxidation. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.1016/j.algal.2021.102476
Počet záznamů: 1