Počet záznamů: 1
Epigenetic distribution of recombinant plant chromosome fragments in a human–arabidopsis hybrid cell line
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SYSNO ASEP 0547644 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Epigenetic distribution of recombinant plant chromosome fragments in a human–arabidopsis hybrid cell line Tvůrce(i) Liaw, Y. M. (JP)
Liu, Y. (AU)
Teo, C. H. (MY)
Cápal, Petr (UEB-Q) RID, ORCID
Wada, N. (JP)
Fukui, K. (JP)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Ohmido, N. (JP)Celkový počet autorů 8 Číslo článku 5426 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 11 (2021)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Arabidopsis genome ; DNA methylation ; Epigenome ; Gene expression ; Human–plant hybrid cell line ; Whole-genome bisulfite sequencing (WGBS) Obor OECD Biochemistry and molecular biology CEP EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000660187400001 EID SCOPUS 85106293642 DOI 10.3390/ijms22115426 Anotace Methylation systems have been conserved during the divergence of plants and animals, although they are regulated by different pathways and enzymes. However, studies on the interactions of the epigenomes among evolutionarily distant organisms are lacking. To address this, we studied the epigenetic modification and gene expression of plant chromosome fragments (~30 Mb) in a human–Arabidopsis hybrid cell line. The whole-genome bisulfite sequencing results demonstrated that recombinant Arabidopsis DNA could retain its plant CG methylation levels even without functional plant methyltransferases, indicating that plant DNA methylation states can be maintained even in a different genomic background. The differential methylation analysis showed that the Arabidopsis DNA was undermethylated in the centromeric region and repetitive elements. Several Arabidopsis genes were still expressed, whereas the expression patterns were not related to the gene function. We concluded that the plant DNA did not maintain the original plant epigenomic landscapes and was under the control of the human genome. This study showed how two diverging genomes can coexist and provided insights into epigenetic modifications and their impact on the regulation of gene expressions between plant and animal genomes. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.3390/ijms22115426
Počet záznamů: 1