Počet záznamů: 1
The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System
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SYSNO ASEP 0542141 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System Tvůrce(i) Blanquer, Andreu (FGU-C) RID, ORCID
Musílková, Jana (FGU-C) RID, ORCID
Filová, Elena (FGU-C) RID, ORCID
Táborská, Johanka (UMCH-V)
Brynda, Eduard (UMCH-V) RID
Riedel, Tomáš (UMCH-V) RID, ORCID
Klápšťová, A. (CZ)
Jenčová, V. (CZ)
Müllerová, J. (CZ)
Kuželová Košťáková, E. (CZ)
Procházková, R. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDČíslo článku 457 Zdroj.dok. Nanomaterials. - : MDPI
Roč. 11, č. 2 (2021)Poč.str. 26 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova electrospun nanofibre ; skin wound healing ; keratinocytes ; platelet lysate ; fibrin ; endothelial cells ; in vitro co-culture system Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Biomaterials (as related to medical implants, devices, sensors) Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Biotechnologie a bionika CEP LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NV18-01-00332 GA MZd - Ministerstvo zdravotnictví Výzkumná infrastruktura Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; UMCH-V - RVO:61389013 UT WOS 000622921700001 EID SCOPUS 85100639107 DOI 10.3390/nano11020457 Anotace Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research of new therapeutic approaches to wound healing includes the development of nanofibrous meshes and the use of platelet lysate (PL) to stimulate skin regeneration. This study considers a combination of a degradable electrospun nanofibrous blend of poly(L-lactide-co-epsilon-caprolactone) and poly(epsilon-caprolactone) (PLCL/PCL) membranes (NF) and fibrin loaded with various concentrations of PL aimed at the development of bioactive skin wound healing dressings. The cytocompatibility of the NF membranes, as well as the effect of PL, was evaluated in both monocultures and co-cultures of human keratinocytes and human endothelial cells. We determined that the keratinocytes were able to adhere on all the membranes, and their increased proliferation and differentiation was observed on the membranes that contained fibrin with at least 50% of PL (Fbg + PL) after 14 days. With respect to the co-culture experiments, the membranes with fibrin with 20% of PL were observed to enhance the metabolic activity of endothelial cells and their migration, and the proliferation and differentiation of keratinocytes. The results suggest that the newly developed NF combined with fibrin and PL, described in the study, provides a promising dressing for chronic wound healing purposes. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2079-4991/11/2/457
Počet záznamů: 1