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The Role of miR-21 in Osteoblasts-Osteoclasts Coupling In Vitro
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SYSNO ASEP 0540779 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Role of miR-21 in Osteoblasts-Osteoclasts Coupling In Vitro Tvůrce(i) Smieszek, A. (PL)
Marcinkowska, K. (PL)
Pielok, A. (PL)
Sikora, M. (PL)
Valihrach, Lukáš (BTO-N) RID, ORCID
Marycz, K. (PL)Celkový počet autorů 6 Číslo článku 479 Zdroj.dok. Cells. - : MDPI
Roč. 9, č. 2 (2020)Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova miR-21-5p ; differentiation ; osteogenesis ; precursor cells Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP GA18-21942S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000521944900220 DOI 10.3390/cells9020479 Anotace MiR-21 is being gradually more and more recognized as a molecule regulating bone tissue homeostasis. However, its function is not fully understood due to the dual role of miR-21 on bone-forming and bone-resorbing cells. In this study, we investigated the impact of miR-21 inhibition on pre-osteoblastic cells differentiation and paracrine signaling towards pre-osteoclasts using indirect co-culture model of mouse pre-osteoblast (MC3T3) and pre-osteoclast (4B12) cell lines. The inhibition of miR-21 in MC3T3 cells (MC3T3(inh21)) modulated expression of genes encoding osteogenic markers including collagen type I (Coll-1), osteocalcin (Ocl), osteopontin (Opn), and runt-related transcription factor 2 (Runx-2). Inhibition of miR-21 in osteogenic cultures of MC3T3 also inflected the synthesis of OPN protein which is essential for proper mineralization of extracellular matrix (ECM) and anchoring osteoclasts to the bones. Furthermore, it was shown that in osteoblasts miR-21 regulates expression of factors that are vital for survival of pre-osteoclast, such as receptor activator of nuclear factor kappa B ligand (RANKL). The pre-osteoclast cultured with MC3T3(inh21) cells was characterized by lowered expression of several markers associated with osteoclasts' differentiation, foremost tartrate-resistant acid phosphatase (Trap) but also receptor activator of nuclear factor-kappa B ligand (Rank), cathepsin K (Ctsk), carbonic anhydrase II (CaII), and matrix metalloproteinase (Mmp-9). Collectively, our data indicate that the inhibition of miR-21 in MC3T3 cells impairs the differentiation and ECM mineralization as well as influences paracrine signaling leading to decreased viability of pre-osteoclasts. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/2073-4409/9/2/479
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