Počet záznamů: 1
Enhancement of the biological autoluminescence by mito-liposomal gold nanoparticle nanocarriers
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SYSNO ASEP 0538002 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Enhancement of the biological autoluminescence by mito-liposomal gold nanoparticle nanocarriers Tvůrce(i) Sardarabadi, H. (IR)
Chafai, Djamel Eddine (URE-Y)
Gheybi, F. (IR)
Sasanpour, P. (IR)
Rafii-Tabar, H. (IR)
Cifra, Michal (URE-Y) RID, ORCID, SAICelkový počet autorů 6 Číslo článku 111812 Zdroj.dok. Journal of Photochemistry and Photobiology. B - Biology Section. - : Elsevier - ISSN 1011-1344
Roč. 204, March (2020)Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Biological autoluminescence signal enhancement ; Electron-excited biomolecules ; Gold nanoparticles ; Mitochondrial-liposomal nanocarrier Vědní obor RIV JA - Elektronika a optoelektronika, elektrotechnika Obor OECD Electrical and electronic engineering CEP GA18-23597S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora URE-Y - RVO:67985882 UT WOS 000518871200029 EID SCOPUS 85079147459 DOI 10.1016/j.jphotobiol.2020.111812 Anotace One of the most important barriers to the detection of the biological autoluminescence (BAL) from biosystems using a non-invasive monitoring approach, in both the in vivo and the in vitro applications, is its very low signal intensity ( < 1000 photons/s/cm(2)). Experimental studies have revealed that the formation of electron excited species, as a result of reactions of biomolecules with reactive oxygen species (ROS), is the principal biochemical source of the BAL which occurs during the cell metabolism. Mitochondria, as the most important organelles involved in oxidative metabolism, are considered to be the main intracellular BAL source. Hence, in order to achieve the BAL enhancement via affecting the mitochondria, we prepared a novel mitochondrial-liposomal nanocarrier with two attractive features including the intra-liposomal gold nanoparticle synthesizing ability and the mitochondria penetration capability. The results indicate that these nanocarriers (with the average size of 131.1 +/- 20.1 nm) are not only able to synthesize the gold nanoparticles within them (with the average size of 15 nm) and penetrate into the U2OS cell mitochondria, but they are also able to amplify the BAL signals. Our results open new possibilities for the use of biological autoluminescence as a non-invasive and label-free monitoring method in nanomedicine and biotechnology Pracoviště Ústav fotoniky a elektroniky Kontakt Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Rok sběru 2021 Elektronická adresa https://doi.org/10.1016/j.jphotobiol.2020.111812
Počet záznamů: 1