Počet záznamů: 1
Binding of Kingella kingae RtxA Toxin Depends on Cell Surface Oligosaccharides, but Not on beta(2) Integrins
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SYSNO ASEP 0536763 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Binding of Kingella kingae RtxA Toxin Depends on Cell Surface Oligosaccharides, but Not on beta(2) Integrins Tvůrce(i) Rahman, Waheed Ur (MBU-M) ORCID
Osičková, Adriana (MBU-M) RID, ORCID
Klímová, Nela (MBU-M) ORCID
Lora, J. (US)
Balashova, N. (US)
Osička, Radim (MBU-M) RID, ORCIDČíslo článku 9092 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 23 (2020)Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova integrins ; Kingella kingae ; oligosaccharides Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA18-18079S GA ČR - Grantová agentura ČR LM2018133 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000597593100001 EID SCOPUS 85097035918 DOI 10.3390/ijms21239092 Anotace The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections. K. kingae secretes an RtxA toxin that belongs to a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens. Recently, we demonstrated that membrane cholesterol facilitates interaction of RtxA with target cells, but other cell surface structures potentially involved in toxin binding to cells remain unknown. We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells. Consequently, the deglycosylated cells were more resistant to cytotoxic activity of RtxA. Moreover, experiments on cells expressing or lacking cell surface integrins of the beta(2) family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the beta(2) integrins. Our results, hence, show that RtxA binds cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted beta(2) integrins. This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/1422-0067/21/23/9092
Počet záznamů: 1