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Structure-to-efficacy relationship of HPMA-based nanomedicines: the tumor spheroid penetration study
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SYSNO ASEP 0536604 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Structure-to-efficacy relationship of HPMA-based nanomedicines: the tumor spheroid penetration study Tvůrce(i) Kudláčová, Júlia (UMCH-V) RID, ORCID
Kotrchová, Lenka (UMCH-V) RID, ORCID
Kostka, Libor (UMCH-V) RID, ORCID
Randárová, Eva (UMCH-V) RID
Filipová, Marcela (UMCH-V) RID, ORCID
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
Fang, J. (JP)
Etrych, Tomáš (UMCH-V) RID, ORCIDČíslo článku 1242 Zdroj.dok. Pharmaceutics. - : MDPI
Roč. 12, č. 12 (2020), s. 1-15Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova HPMA polymers ; pirarubicin ; tumor spheroids Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP LTAUSA18083 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA19-01417S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000602306000001 EID SCOPUS 85098233087 DOI 10.3390/pharmaceutics12121242 Anotace Nanomedicines are a novel class of therapeutics that benefit from the nano dimensions of the drug carrier. These nanosystems are highly advantageous mainly within cancer treatment due to their enhanced tumor accumulation. Monolayer tumor cells frequently used in routine preclinical assessment of nanotherapeutics do not have a spatial structural architecture that allows the investigation of the penetration of nanomedicines to predict their behavior in real tumor tissue. Therefore, tumor spheroids from colon carcinoma C26 cells and glioblastoma U87-MG cells were used as 3D in vitro models to analyze the effect of the inner structure, hydrodynamic size, dispersity, and biodegradability of N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-based nanomedicines carrying anticancer drug pirarubicin (THP) on the penetration within spheroids. While almost identical penetration through spheroids of linear and star-like copolymers and also their conjugates with THP was observed, THP penetration after nanomedicines application was considerably deeper than for the free THP, thus proving the benefit of polymer carriers. The cytotoxicity of THP-polymer nanomedicines against tumor cell spheroids was almost identical as for the free THP, whereas the 2D cell cytotoxicity of these nanomedicines is usually lower. The nanomedicines thus proved the enhanced efficacy within the more realistic 3D tumor cell spheroid system. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/1999-4923/12/12/1242
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