Počet záznamů: 1
Spirochetes isolated from arthropods constitute a novel genus Entomospira genus novum within the order Spirochaetales
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SYSNO ASEP 0533116 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Spirochetes isolated from arthropods constitute a novel genus Entomospira genus novum within the order Spirochaetales Tvůrce(i) Graña‑Miraglia, L. (MX)
Šikutová, Silvie (UBO-W) RID, SAI, SAI, ORCID
Vancová, Marie (BC-A) RID, ORCID
Bílý, Tomáš (BC-A) ORCID
Fingerle, V. (DE)
Sing, A. (DE)
Castillo‑Ramírez, S. (MX)
Margos, G. (DE)
Rudolf, Ivo (UBO-W) RID, ORCID, SAICelkový počet autorů 9 Číslo článku 17053 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, č. 1 (2020)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Borrelia burgdorferi ; lyme disease ; etiologic agent ; mosquitos ; reclassification Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Genetika a molekulární biologie CEP LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UBO-W - RVO:68081766 ; BC-A - RVO:60077344 UT WOS 000582686700003 EID SCOPUS 85092468734 DOI 10.1038/s41598-020-74033-9 Anotace The psychrophilic (cold-loving) fungus Pseudogymnoascus destructans was discovered more than a decade ago to be the pathogen responsible for white-nose syndrome, an emerging disease of North American bats causing unprecedented population declines. The same species of fungus is found in Europe but without associated mortality in bats. We found P. destructans was infected with a mycovirus [named Pseudogymnoascus destructans partitivirus 1 (PdPV-1)]. The virus is bipartite, containing two double-stranded RNA (dsRNA) segments designated as dsRNA1 and dsRNA2. The cDNA sequences revealed that dsRNA1 dsRNA is 1,683 bp in length with an open reading frame (ORF) that encodes 539 amino acids (molecular mass of 62.7 kDa), dsRNA2 dsRNA is 1,524 bp in length with an ORF that encodes 434 amino acids (molecular mass of 46.9 kDa). The dsRNA1 ORF contains motifs representative of RNA-dependent RNA polymerase (RdRp), whereas the dsRNA2 ORF sequence showed homology with the putative capsid proteins (CPs) of mycoviruses. Phylogenetic analyses with PdPV-1 RdRp and CP sequences indicated that both segments constitute the genome of a novel virus in the family Partitiviridae. The purified virions were isometric with an estimated diameter of 33 nm. Reverse transcription PCR (RT-PCR) and sequencing revealed that all US isolates and a subset of Czech Republic isolates of P. destructans were infected with PdPV-1. However, PdPV-1 appears to be not widely dispersed in the fungal genus Pseudogymnoascus, as non-pathogenic fungi P. appendiculatus (1 isolate) and P. roseus (6 isolates) tested negative. P. destructans PdPV-1 could be a valuable tool to investigate fungal biogeography and the host–pathogen interactions in bat WNS. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2021 Elektronická adresa https://www.nature.com/articles/s41598-020-74033-9.pdf
Počet záznamů: 1