Počet záznamů: 1
Community dynamics and function of algae and bacteria during winter in central European great lakes
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SYSNO ASEP 0532694 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Community dynamics and function of algae and bacteria during winter in central European great lakes Tvůrce(i) Bullerjahn, G. S. (US)
McKay, R. M. L. (US)
Bernát, Gábor (MBU-M) ORCID
Prášil, Ondřej (MBU-M) RID, ORCID
Voros, L. (HU)
Palffy, K. (HU)
Tugyi, N. (HU)
Somogyi, B. (HU)Zdroj.dok. Journal of Great Lakes Research. - : Elsevier - ISSN 0380-1330
Roč. 46, č. 4 (2020), s. 732-740Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova ice ; diatom ; phytoplankton ; assemblages ; erie Vědní obor RIV DA - Hydrologie a limnologie Obor OECD Marine biology, freshwater biology, limnology CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EE2.3.20.0203 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED2.1.00/19.0392 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000556753300005 EID SCOPUS 85069555303 DOI 10.1016/j.jglr.2019.07.002 Anotace Abundant phytoplankton and bacteria were identified by microscopy and high-throughput 16S rRNA tag Illumina sequencing of samples from water- and ice phases collected during winter at two central European Great Lakes, Balaton and Ferto (Neusiedlersee). Bacterial reads at all sites were dominated (>85%) by Bacteroidetes and Proteobacteria. Amongst phototrophs, microscopy and 16S sequencing revealed that both phytoplankton and cyanobacteria were represented, with a median of 1500 cyanobacterial sequence reads amongst 13 samples analyzed. The sequence analysis compared replicate Balaton and Ferto ice and water samples with an outgroup from three Hungarian soda lakes. In particular, both water and ice from Ferto contained high contributions from cyanobacteria. Two percent of total reads identified to the level of family in water at Nab were dominated by a single operational taxonomic unit (OTU) of a cyanobacterium within the Rivulariaceae, which was largely absent from ice. Conversely, ice samples from both lakes yielded an abundant OTU assigned to a Flavobacterium sp. known to be associated with freshwater ice. Principal Coordinates Analysis (PCoA) revealed that the ice communities from all sites were similar to one another, and that the water communities did not cluster together. Fluorescence emission spectra obtained at 77 K confirmed the presence of intact cyanobacteria in Fento water and ice. Photosynthetic characterization of phototrophs resident in water and ice analyzed by assay of acid-stable photosynthetic (HCO3-)-C-14 incorporation showed that communities from both phases were photosynthetically active, thus adding to growing recognition of ice-covered lakes as viable habitat for phototrophs. (C) 2019 International Association for Great lakes Research. Published by Elsevier B.V. All rights reserved. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://www.sciencedirect.com/science/article/abs/pii/S0380133019301182
Počet záznamů: 1