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PHA granules help bacterial cells to preserve cell integrity when exposed to sudden osmotic imbalances
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SYSNO ASEP 0517412 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název PHA granules help bacterial cells to preserve cell integrity when exposed to sudden osmotic imbalances Tvůrce(i) Sedláček, P. (CZ)
Slaninova, E. (CZ)
Koller, M. (AT)
Nebesářová, Jana (BC-A) RID, ORCID
Marová, I. (CZ)
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Obruča, S. (CZ)Celkový počet autorů 7 Zdroj.dok. New Biotechnology. - : Elsevier - ISSN 1871-6784
Roč. 49, MAR 25 2019 (2019), s. 129-136Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova stress ; bioremediation ; Polyhydroxyalkanoates ; Osmotic imbalance ; Hypotonic lysis ; Cupriavidus necator ; Halomonas halophila ; Halophiles Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) Vědní obor RIV – spolupráce Ústav přístrojové techniky - Elektronika a optoelektronika, elektrotechnika CEP GA15-20645S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BC-A - RVO:60077344 ; UPT-D - RVO:68081731 UT WOS 000454542600017 EID SCOPUS 85056350852 DOI 10.1016/j.nbt.2018.10.005 Anotace Polyhydroxyalkanoates (PHA) are microbial polyesters which accumulate as intracellular granules in numerous prokaryotes and mainly serve as storage materials, beyond this primary function, PHA also enhance the robustness of bacteria against various stress factors. We have observed that the presence of PHA in bacterial cells substantially enhances their ability to maintain cell integrity when suddenly exposed to osmotic imbalances. In the case of the non-halophilic bacterium Cupriavidus necator, the presence of PHA decreased plasmolysis-induced cytoplasmic membrane damage during osmotic up-shock, which subsequently enabled the cells to withstand subsequent osmotic downshock. In contrast, sudden induction of osmotic up-and subsequent down-shock resulted in massive hypotonic lysis of non-PHA containing cells as determined by Transmission Electron Microscopy and Thermogravimetrical Analysis. Furthermore, a protective effect of PHA against hypotonic lysis was also observed in the case of the halophilic bacterium Halomonas halophila, here, challenged PHA-rich cells were capable of retaining cell integrity more effectively than their PHA-poor counterparts. Hence, it appears that the fact that PHA granules, as an added value to their primary storage function, protect halophiles from the harmful effect of osmotic down-shock might explain why PHA accumulation is such a common feature among halophilic prokaryotes. The results of this study, apart from their fundamental importance, are also of practical biotechnological significance: because PHA-rich bacterial cells are resistant to osmotic imbalances, they could be utilized in in-situ bioremediation technologies or during enrichment of mixed microbial consortia in PHA producers under conditions of fluctuating salinity. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S1871678418305119?via%3Dihub
Počet záznamů: 1