Počet záznamů: 1
Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities
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SYSNO ASEP 0506620 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities Tvůrce(i) Bunina, D. (DE)
Štefl, Martin (UFCH-W)
Huber, F. (DE)
Khmelinskii, A. (DE)
Meurer, M. (DE)
Barry, J.D. (DE)
Kats, I. (DE)
Kirrmaier, D. (DE)
Huber, W. (DE)
Knop, M. (DE)Zdroj.dok. Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 45, č. 19 (2017), s. 11144-11158Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova saccharomyces-cerevisiae ; budding yeast ; pervasive transcription ; noncoding transcription ; bidirectional promoters ; nucleosome occupancy ; genome ; heterogeneity ; proteins ; identification Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry Způsob publikování Open access Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000414552300022 EID SCOPUS 85037984799 DOI 10.1093/nar/gkx737 Anotace Pervasive transcription of genomes generates multiple classes of non-coding RNAs. One of these classes are stable long non-coding RNAs which overlap coding genes in antisense direction (asRNAs). The function of such asRNAs is not fully understood but several cases of antisense-dependent gene expression regulation affecting the overlapping genes have been demonstrated. Using high-throughput yeast genetics and a limited set of four growth conditions we previously reported a regulatory function for similar to 25% of asRNAs, most of which repress the expression of the sense gene. To further explore the roles of asRNAs we tested more conditions and identified 15 conditionally antisense-regulated genes, 6 of which exhibited antisense-dependent enhancement of gene expression. We focused on the sporulation-specific gene SPS100, which becomes upregulated upon entry into starvation or sporulation as a function of the antisense transcript SUT169. We demonstrate that the antisense effect is mediated by its 3 ' intergenic region (3 '-IGR) and that this regulation can be transferred to other genes. Genetic analysis revealed that SUT169 functions by changing the relative expression of SPS100 mRNA isoforms from a short and unstable transcript to a long and stable species. These results suggest a novel mechanism of antisense-dependent gene regulation via mRNA isoform switching. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2020 Elektronická adresa http://hdl.handle.net/11104/0297831
Počet záznamů: 1