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Comparison of enzymatic activities and proteomic profiles of Butyrivibrio fibrisolvens grown on different carbon sources
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SYSNO ASEP 0506140 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Comparison of enzymatic activities and proteomic profiles of Butyrivibrio fibrisolvens grown on different carbon sources Tvůrce(i) Sechovcová, Hana (UZFG-Y) ORCID
Kulhavá, Lucie (FGU-C) ORCID
Fliegerová, Kateřina (UZFG-Y) RID, ORCID
Trundová, Mária (BTO-N)
Morais, Daniel (MBU-M) ORCID
Mrázek, Jakub (UZFG-Y) RID, ORCID
Kopečný, Jan (UZFG-Y) RID, ORCIDČíslo článku 2 Zdroj.dok. Proteome Science. - : BioMed Central
Roč. 17, JUN 1 (2019)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Butyrivibrio fibrisolvens ; proteomics ; rumen ; carbon sources Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Biotechnologický ústav - Genetika a molekulární biologie CEP GA16-12431S GA ČR - Grantová agentura ČR EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 ; FGU-C - RVO:67985823 ; BTO-N - RVO:86652036 ; MBU-M - RVO:61388971 UT WOS 000469813700001 EID SCOPUS 85066870472 DOI 10.1186/s12953-019-0150-3 Anotace Background: The rumen microbiota is one of the most complex consortia of anaerobes, involving archaea, bacteria, protozoa, fungi and phages. They are very effective at utilizing plant polysaccharides, especially cellulose and hemicelluloses. The most important hemicellulose decomposers are clustered with the genus Butyrivibrio. As the related species differ in their range of hydrolytic activities and substrate preferences, Butyrivibrio fibrisolvens was selected as one of the most effective isolates and thus suitable for proteomic studies on substrate comparisons in the extracellular fraction. The B. fibrisolvens genome is the biggest in the butyrivibria cluster and is focused on environmental information processing and carbohydrate metabolism.
Methods: The study of the effect of carbon source on B. fibrisolvens 3071 was based on cultures grown on four substrates: xylose, glucose, xylan, xylan with 25% glucose. The enzymatic activities were studied by spectrophotometric and zymogram methods. Proteomic study was based on genomics, 2D electrophoresis and nLC/MS (Bruker Daltonics) analysis.
Results: Extracellular beta-endoxylanase as well as xylan beta-xylosidase activities were induced with xylan. The presence of the xylan polymer induced hemicellulolytic enzymes and increased the protein fraction in the interval from 40 to 80kDa. 2D electrophoresis with nLC/MS analysis of extracellular B. fibrisolvens 3071 proteins found 14 diverse proteins with significantly different expression on the tested substrates.
Conclusion: The comparison of four carbon sources resulted in the main significant changes in B. fibrisolvens proteome occurring outside the fibrolytic cluster of proteins. The affected proteins mainly belonged to the glycolysis and protein synthesis cluster.Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://proteomesci.biomedcentral.com/articles/10.1186/s12953-019-0150-3
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