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Enantiomeric separation of triacylglycerols containing very long chain fatty acids
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SYSNO ASEP 0490505 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Enantiomeric separation of triacylglycerols containing very long chain fatty acids Tvůrce(i) Řezanka, Tomáš (MBU-M) ORCID
Kolouchová, I. (CZ)
Nedbalová, L. (CZ)
Sigler, Karel (MBU-M) RIDZdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1557, JUL 6 (2018), s. 9-19Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Triacylglycerols ; Very long chain fatty acids ; Very long-chain polyunsaturated fatty acids Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA17-00027S GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000433651500002 EID SCOPUS 85046689823 DOI 10.1016/j.chroma.2018.04.064 Anotace Enantiomers of triacylglycerols (TAGs) containing any combination of very long chain fatty acids (VLCFAs) and/or very long chain polyunsaturated fatty acids (VLCPUFAs) with diolein, dilinolein and didocosahexaenoin were synthesized. Gradient non-aqueous reversed-phase high-performance liquid chromatography/high resolution atmospheric pressure chemical ionization-tandem mass spectrometry (NARP-HPLC/HRMS2-APCI) and chiral liquid chromatography were used for the separation and identification of molecular species of these TAGs. Further, NARP-LC and chiral LC were used to separate natural mixtures of TAGs obtained from four natural sources, i.e. ximenia oil (Ximenia americana), green alga (Botryococcus braunii), breweris yeast (Saccharomyces pastorianus) and a dinoflagellate (Amphidinium carterae). The ratio of regioisomers and enantiomers in individual samples was determined and a hypothesis has been confirmed on the biosynthetic pathway of natural TAGs, which is based on the preferential representation of VLCFAs and VLCPUFAs in the sn-1 position of the glycerol backbone. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
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