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Biopolymer of Dictyosphaerium chlorelloides chemical characterization and biological effects

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    0490105 - BÚ 2019 RIV GB eng J - Článek v odborném periodiku
    Halaj, M. - Paulovičová, E. - Paulovicova, L. - Jantová, S. - Cepák, Vladislav - Lukavský, Jaromír - Capek, P.
    Biopolymer of Dictyosphaerium chlorelloides chemical characterization and biological effects.
    International Journal of Biological Macromolecules. Roč. 113, JUL 1 2018 (2018), s. 1248-1257. ISSN 0141-8130. E-ISSN 1879-0003
    Grant CEP: GA TA ČR TE01020080
    Výzkumný záměr: CEZ:AV0Z60050516
    Institucionální podpora: RVO:67985939
    Klíčová slova: Green microalgae * Extracellular polysaccharide * Cytokines
    Obor OECD: Polymer science
    Impakt faktor: 4.784, rok: 2018

    Some microalgae are producers of high-value phytochemicals of interest for industrial applications. Cultivation of Dictyosphaerium chlorelloides microalgae yielded about 1 g/L of a viscous exobiopolymer with a high molecular weight (M-p, = 9.6 x 10(5) g/mol). Its proteoglycan character was confirmed by the presence of proteins and carbohydrates. Dominant sugars of the biopolymer were galactose and its methyl-derivatives (similar to 42 wt%), rhamnose (18 wt%), mannose (14 wt%) and unidentified methyl-hexoses (12 wt%). Besides, glucose and 3-0-methyl derivative (8 wt%), xylose and 4-0-methyl-derivative (4 wt%), and arabinose (2 wt%) were found. Methylation analysis indicated a complex chemical structure. Immunobiological studies revealed the effective anti-proliferative capacity of the biopolymer in vitro in both cancer and non-cancer cell lines. Based on in vitro exposition using a reconstructed 3-D tissue model, a good bioavailability and a low toxicity of the biopolymer have been found. Exposition of RAW 264.7 macrophages to the biopolymer resulted into significant immunostimulation of pro and anti-inflammatory cytokines, and decreased free radical cell release.
    Trvalý link: http://hdl.handle.net/11104/0284398

     
     
Počet záznamů: 1  

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