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The Arabidopsis thaliana non-specific phospholipase C2 is involved in the response to Pseudomonas syringae attack
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SYSNO ASEP 0488720 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Arabidopsis thaliana non-specific phospholipase C2 is involved in the response to Pseudomonas syringae attack Tvůrce(i) Krčková, Zuzana (UEB-Q) ORCID
Kocourková, Daniela (UEB-Q) ORCID
Daněk, Michal (UEB-Q) ORCID
Brouzdová, Jitka (UEB-Q)
Pejchar, Přemysl (UEB-Q) RID, ORCID
Janda, Martin (UEB-Q) RID, ORCID
Pokotylo, I. (UA)
Ott, P.G. (HU)
Valentová, O. (CZ)
Martinec, Jan (UEB-Q) RID, ORCIDCelkový počet autorů 10 Zdroj.dok. Annals of Botany. - : Oxford University Press - ISSN 0305-7364
Roč. 121, č. 2 (2018), s. 297-310Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Arabidopsis thaliana ; effector-triggered immunity ; flagellin ; MAMP-triggered immunity ; non-specific phospholipase C ; phosphatidylcholine-specific phospholipase C ; Pseudomonas syringae ; reactive oxygen species Vědní obor RIV ED - Fyziologie Obor OECD Plant sciences, botany CEP GAP501/12/1942 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000425623800010 EID SCOPUS 85042215236 DOI 10.1093/aob/mcx160 Anotace Background and Aims: The non-specific phospholipase C (NPC) is a new member of the plant phospholipase family that reacts to abiotic environmental stresses, such as phosphate deficiency, high salinity, heat and aluminium toxicity, and is involved in root development, silicon distribution and brassinolide signalling. Six NPC genes (NPC1-NPC6) are found in the Arabidopsis genome. The NPC2 isoform has not been experimentally characterized so far. Methods: The Arabidopsis NPC2 isoform was cloned and heterologously expressed in Escherichia coli. NPC2 enzyme activity was determined using fluorescent phosphatidylcholine as a substrate. Tissue expression and subcellular localization were analysed using GUS- and GFP-tagged NPC2. The expression patterns of NPC2 were analysed via quantitative real-time PCR. Independent homozygous transgenic plant lines overexpressing NPC2 under the control of a 35S promoter were generated, and reactive oxygen species were measured using a luminol-based assay. Key Results: The heterologously expressed protein possessed phospholipase C activity, being able to hydrolyse phosphatidylcholine to diacylglycerol. NPC2 tagged with GFP was predominantly localized to the Golgi apparatus in Arabidopsis roots. The level of NPC2 transcript is rapidly altered during plant immune responses and correlates with the activation of multiple layers of the plant defence system. Transcription of NPC2 decreased substantially after plant infiltration with Pseudomonas syringae, flagellin peptide flg22 and salicylic acid treatments and expression of the effector molecule AvrRpm1. The decrease in NPC2 transcript levels correlated with a decrease in NPC2 enzyme activity. NPC2-overexpressing mutants showed higher reactive oxygen species production triggered by flg22. Conclusions: This first experimental characterization of NPC2 provides new insights into the role of the nonspecific phospholipase C protein family. The results suggest that NPC2 is involved in the response of Arabidopsis to P. Syringae attack. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
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