Počet záznamů: 1
Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings
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SYSNO ASEP 0486146 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings Tvůrce(i) Filová, Elena (FGU-C) RID, ORCID
Vandrovcová, Marta (FGU-C) RID, ORCID
Jelínek, Miroslav (FZU-D) RID, ORCID
Zemek, Josef (FZU-D) RID, ORCID
Houdková, Jana (FZU-D) RID, ORCID
Remsa, Jan (FZU-D) RID, ORCID
Kocourek, Tomáš (FZU-D) RID, ORCID, SAI
Staňková, Ľubica (FGU-C) RID, ORCID
Bačáková, Lucie (FGU-C) RID, ORCIDČíslo článku 17 Zdroj.dok. Journal of Materials Science-Materials in Medicine. - : Springer - ISSN 0957-4530
Roč. 28, č. 1 (2017)Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova osteocalcin ; osteogenic differentiation ; hexavalent chromium ; focal adhesion contact ; cell spreading area Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Biomaterials (as related to medical implants, devices, sensors) CEP GA15-05864S GA ČR - Grantová agentura ČR GA14-04790S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 ; FZU-D - RVO:68378271 UT WOS 000391394200017 EID SCOPUS 85006705679 DOI 10.1007/s10856-016-5830-2 Anotace Diamond-like carbon (DLC) thin films are promising for use in coating orthopaedic, dental and cardiovascular implants. The problem of DLC layers lies in their weak layer adhesion to metal implants. Chromium is used as a dopant for improving the adhesion of DLC films. Cr-DLC layers were prepared by a hybrid technology, using a combination of pulsed laser deposition (PLD) from a graphite target and magnetron sputtering. Depending on the deposition conditions, the concentration of Cr in the DLC layers moved from zero to 10.0 at.%. The effect of DLC layers with 0.0, 0.9, 1.8, 7.3, 7.7 and 10.0 at.% Cr content on the adhesion and osteogenic differentiation of human osteoblast-like Saos-2 cells was assessed in vitro. The DLC samples that contained 7.7 and 10.0 at.% of Cr supported cell spreading on day 1 after seeding. On day three after seeding, the most apparent vinculin-containing focal adhesion plaques were also found on samples with higher concentrations of chromium. On the other hand, the expression of type I collagen and alkaline phosphatase at the mRNA and protein level was the highest on Cr-DLC samples with a lower concentration of Cr (0-1.8 at.%). We can conclude that higher concentrations of chromium supported cell adhesion, however DLC and DLC doped with a lower concentration of chromium supported osteogenic cell differentiation. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
Počet záznamů: 1