Transgenerational inheritance of susceptibility to diabetes-induced male subfertility

0482594 - BTÚ 2018 RIV GB eng J - Článek v odborném periodiku
Pavlínková, Gabriela - Margaryan, Hasmik - Žatecká, Eva - Valášková, Eliška - Elzeinová, Fatima - Kubátová, Alena - Bohuslavová, Romana - Pěknicová, Jana
Transgenerational inheritance of susceptibility to diabetes-induced male subfertility.
Scientific Reports. Roč. 7, JUL 10 2017 (2017), č. článku 4940. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA MZd(CZ) NV15-30880A; GA ČR(CZ) GA16-06825S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: HIGH-FAT DIET * MALE-INFERTILITY * GENE-EXPRESSION * SPERM PARAMETERS
Obor OECD: Endocrinology and metabolism (including diabetes, hormones)
Impakt faktor: 4.122, rok: 2017

Male infertility is a worldwide problem associated with genetic background, environmental factors, and diseases. One of the suspected contributing factors to male infertility is diabetes mellitus. We investigated the molecular and morphological changes in sperms and testicular tissue of diabetic males. The study was performed in streptozotocin-induced type 1 diabetes mouse model. Diabetes decreased sperm concentration and viability and increased sperm apoptosis. Changes in protamine 1/protamine 2 ratio indicated reduced sperm quality. The testicular tissue of diabetic males showed significant tissue damage, disruption of meiotic progression, and changes in the expression of genes encoding proteins important for spermiogenesis. Paternal diabetes altered sperm quality and expression pattern in the testes in offspring of two subsequent generations. Our study revealed that paternal diabetes increased susceptibility to infertility in offspring through gametic alternations. Our data also provide a mechanistic basis for transgenerational inheritance of diabetes-associated pathologies since protamines may be involved in epigenetic regulations.
Trvalý link: http://hdl.handle.net/11104/0278004