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The presence of PHB granules in cytoplasm protects non-halophilic bacterial cells against the harmful impact of hypertonic environments
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SYSNO ASEP 0481655 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The presence of PHB granules in cytoplasm protects non-halophilic bacterial cells against the harmful impact of hypertonic environments Tvůrce(i) Obruča, S. (CZ)
Sedláček, P. (CZ)
Mravec, F. (CZ)
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Nebesářová, Jana (BC-A) RID, ORCID
Samek, Ota (UPT-D) RID, ORCID, SAI
Kučera, D. (CZ)
Benešová, P. (CZ)
Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Milerová, M. (CZ)
Márová, I. (CZ)Celkový počet autorů 11 Zdroj.dok. New Biotechnology. - : Elsevier - ISSN 1871-6784
Roč. 39, OCT (2017), s. 68-80Poč.str. 13 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Poly(3-hydroxybutyrate) ; PHB ; cupriavidus necator ; hyperosmotic conditions ; plasmolysis ; stress conditions Vědní obor RIV BH - Optika, masery a lasery Obor OECD Electrical and electronic engineering Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Biochemie CEP GA15-20645S GA ČR - Grantová agentura ČR Institucionální podpora UPT-D - RVO:68081731 ; BC-A - RVO:60077344 UT WOS 000410912900010 EID SCOPUS 85025814322 DOI 10.1016/j.nbt.2017.07.008 Anotace Numerous prokaryotes accumulate polyhydroxybutyrate (PHB) intracellularly as a storage material. It has also been proposed that PHB accumulation improves bacterial stress resistance. Cupriavidus necator and its PHB non-accumulating mutant were employed to investigate the protective role of PHB under hypertonic conditions. The presence of PHB granules enhanced survival of the bacteria after exposure to hypertonic conditions. Surprisingly, when coping with such conditions, the bacteria did not utilize PHB to harvest carbon or energy, suggesting that, in the osmotic upshock of C. necator, the protective mechanism of PHB granules is not associated with their hydrolysis. The presence of PHB granules influenced the overall properties of the cells, since challenged PHB-free cells underwent massive plasmolysis accompanied by damage to the cell membrane and the leakage of cytoplasm content, while no such effects were observed in PHB containing bacteria. Moreover, PHB granules demonstrated liquid-like properties indicating that they can partially repair and stabilize cell membranes by plugging small gaps formed during plasmolysis. In addition, the level of dehydration and changes in intracellular pH in osmotically challenged cells were less pronounced for PHB-containing cultures, demonstrating the important role of PHB for bacterial survival under hyperosmotic conditions. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2018
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