Počet záznamů: 1
Pigment structure in the violaxanthin-chlorophyll-a-binding protein VCP
- 1.
SYSNO ASEP 0479490 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Pigment structure in the violaxanthin-chlorophyll-a-binding protein VCP Tvůrce(i) Llansola-Portoles, M.J. (FR)
Litvín, Radek (BC-A) RID, ORCID
Ilioaia, C. (FR)
Pascal, A.A. (FR)
Bína, David (BC-A) RID, ORCID
Robert, B. (FR)Celkový počet autorů 6 Zdroj.dok. Photosynthesis Research. - : Springer - ISSN 0166-8595
Roč. 134, č. 1 (2017), s. 51-58Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova resonance raman-spectroscopy ; light-harvesting complex ; cis-trans isomers ; beta-carotene Vědní obor RIV BO - Biofyzika Obor OECD Biophysics CEP GP14-01377P GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000411172800005 EID SCOPUS 85021819252 DOI 10.1007/s11120-017-0407-6 Anotace Resonance Raman spectroscopy was used to evaluate pigment-binding site properties in the violaxanthin-chlorophyll-a-binding protein (VCP) from Nannochloropsis oceanica. The pigments bound to this antenna protein are chlorophyll-a, violaxanthin, and vaucheriaxanthin. The molecular structures of bound Chl-a molecules are discussed with respect to those of the plant antenna proteins LHCII and CP29, the crystal structures of which are known. We show that three populations of carotenoid molecules are bound by VCP, each of which is in an all-trans configuration. We assign the lower-energy absorption transition of each of these as follows. One violaxanthin population absorbs at 485 nm, while the second population is red-shifted and absorbs at 503 nm. The vaucheriaxanthin population absorbs at 525 nm, a position red-shifted by 2138 cm(-1) as compared to isolated vaucheriaxanthin in n-hexane. The red-shifted violaxanthin is slightly less planar than the blue-absorbing one, as observed for the two central luteins in LHCII, and we suggest that these violaxanthins occupy the two equivalent binding sites in VCP at the centre of the cross-brace. The presence of a highly red-shifted vaucheriaxanthin in VCP is reminiscent of the situation of FCP, in which (even more) highly red-shifted populations of fucoxanthin are present. Tuning carotenoids to absorb in the green-yellow region of the visible spectrum appears to be a common evolutionary response to competition with other photosynthetic species in the aquatic environment. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2018
Počet záznamů: 1