Počet záznamů: 1
Differential expression of human gamma-tubulin isotypes during neuronal development and oxidative stress points to a gamma-tubulin-2 prosurvival function
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SYSNO ASEP 0477962 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Differential expression of human gamma-tubulin isotypes during neuronal development and oxidative stress points to a gamma-tubulin-2 prosurvival function Tvůrce(i) Dráberová, Eduarda (UMG-J) RID, ORCID
Sulimenko, Vadym (UMG-J) RID, ORCID
Vinopal, Stanislav (UMG-J)
Sulimenko, Tetyana (UMG-J)
Sládková, Vladimíra (UMG-J)
D'Agostino, L. (US)
Sobol, Margaryta (UMG-J) RID
Hozák, Pavel (UMG-J) RID, ORCID
Kren, L. (US)
Katsetos, C.D. (US)
Dráber, Pavel (UMG-J) RID, ORCIDCelkový počet autorů 11 Zdroj.dok. FASEB Journal. - : Wiley - ISSN 0892-6638
Roč. 31, č. 5 (2017), s. 1828-1846Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova microtubules ; mitochondria ; neuroblastoma differentiation ; neurons Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP LH12050 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NT14467 GA MZd - Ministerstvo zdravotnictví GAP302/12/1673 GA ČR - Grantová agentura ČR GA15-22194S GA ČR - Grantová agentura ČR GA16-25159S GA ČR - Grantová agentura ČR TE01020118 GA TA ČR - Technologická agentura ČR Institucionální podpora UMG-J - RVO:68378050 UT WOS 000399195500007 DOI 10.1096/fj.201600846RR Anotace gamma-Tubulins are highly conserved members of the tubulin superfamily essential for microtubule nucleation. Humans possess 2 gamma-tubulin genes. It is thought that gamma-tubulin-1 represents aubiquitous isotype, whereas gamma-tubulin-2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation. The molecular basis of the purported functional differences between gamma-tubulins is unknown. We report discrimination of human gamma-tubulins according to their electrophoretic and immunochemical properties. In vitro mutagenesis revealed that the differences in electrophoretic mobility originate in the C-terminal regions of the gamma-tubulins. Using epitope mapping, we discovered mouse monoclonal antibodies that can discriminate between human gamma-tubulin isotypes. Real time quantitative RT-PCR and 2-dimensional-PAGE showed that gamma-tubulin-1 is the dominant isotype in fetal neurons. Although gamma-tubulin-2 accumulates in the adult brain, gamma-tubulin-1 remains the major isotype in various brain regions. Localization of gamma-tubulin-1 in mature neurons was confirmed by immunohistochemistry and immunofluorescence microscopy on clinical samples and tissue microarrays. Differentiation of SH-SY5Y human neuroblastoma cells by all-trans retinoic acid, or oxidative stress induced by mitochondrial inhibitors, resulted in upregulation of gamma-tubulin-2, whereas the expression of gamma-tubulin-1 was unchanged. Fractionation experiments and immunoelectron microscopy revealed an association of gamma-tubulins with mitochondrial membranes. These data indicate that in the face of predominant nu-tubulin-1 expression, the accumulation of gamma-tubulin-2 in mature neurons and neuroblastoma cells during oxidative stress may denote a prosurvival role of gamma-tubulin-2 in neurons.-Draberova, E., Sulimenko, V., Vinopal, S., Sulimenko, T., Sladkova, V., D'Agostino, L., Sobol, M., Hozak, P., Kren, L., Katsetos, C. D., Draber, P. Differential expression of human gamma-tubulin isotypes during neuronal development and oxidative stress points to gamma-tubulin-2 prosurvival function. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2018
Počet záznamů: 1