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Protein p53 Binding to Cisplatin-modified DNA Targets Evaluated by Modification-specific Electrochemical Immunoprecipitation Assay
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SYSNO ASEP 0477358 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Protein p53 Binding to Cisplatin-modified DNA Targets Evaluated by Modification-specific Electrochemical Immunoprecipitation Assay Tvůrce(i) Tichý, Vlastimil (BFU-R) RID
Šebest, Peter (BFU-R) ORCID
Orság, Petr (BFU-R) ORCID
Havran, Luděk (BFU-R) RID, ORCID
Pivoňková, Hana (BFU-R) ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDCelkový počet autorů 6 Zdroj.dok. Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 29, č. 2 (2017), s. 319-323Poč.str. 5 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova catalytic hydrogen evolution ; probe ; mechanism ; suggest Vědní obor RIV CG - Elektrochemie Obor OECD Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) CEP GAP206/11/1638 GA ČR - Grantová agentura ČR Institucionální podpora BFU-R - RVO:68081707 UT WOS 000394998000002 DOI 10.1002/elan.201600480 Anotace Studies of protein interactions with chemically modified nucleic acids are of importance in various areas of biomolecular and biomedical research, including investigations of the binding of proteins important in medicine with DNA modified with drugs and diagnostic applications of modified DNAs in biosensing and bioanalysis. Chemical modification of DNA substrates with various species inside or outside specific protein binding sites can affect the protein-DNA recognition. In this paper we present a simple electrochemical immunoprecipitation technique designed for evaluation of the effects of antitumor drug cisplatin on the p53-DNA binding. The cisplatin-DNA adducts are utilized as electroactive labels allowing a facile determination of the p53-bound modified DNA. Effects observed using this technique accord with results of previous biochemical assays. This approach is potentially applicable in studies that deal with the influence of any electroactive DNA modifications on the protein-DNA binding. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2018
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