Počet záznamů: 1
A Comparative Study of Three Different Types of Stem Cells for Treatment of Rat Spinal Cord Injury
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SYSNO ASEP 0476997 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A Comparative Study of Three Different Types of Stem Cells for Treatment of Rat Spinal Cord Injury Tvůrce(i) Růžička, Jiří (UEM-P)
Machová-Urdzíková, Lucia (UEM-P) ORCID
Gillick, J. (US)
Amemori, Takashi (UEM-P) RID
Romanyuk, Nataliya (UEM-P) RID
Kárová, Kristýna (UEM-P) RID
Závišková, Kristýna (UEM-P)
Dubišová, Jana (UEM-P)
Kubinová, Šárka (UEM-P) RID, ORCID
Murali, R. (US)
Syková, Eva (UEM-P) RID
Jhanwar-Uniyal, M. (US)
Jendelová, Pavla (UEM-P) RID, ORCIDZdroj.dok. Cell Transplantation. - : Sage - ISSN 0963-6897
Roč. 26, č. 4 (2017), s. 585-603Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova spinal cord injury (SCI) ; iPSC-derived human neural progenitors ; Inflammatory response Human fetal neural stem cells Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology CEP GA13-00939S GA ČR - Grantová agentura ČR 7F14057 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 UT WOS 000400124700006 EID SCOPUS 85017598489 DOI 10.3727/096368916X693671 Anotace Three different sources of human stem cells-bone marrow-derived mesenchymal stem cells (BM-MSCs), neural progenitors (NPs) derived from immortalized spinal fetal cell line (SPC-01), and induced pluripotent stem cells (iPSCs)-were compared in the treatment of a balloon-induced spinal cord compression lesion in rats. One week after lesioning, the rats received either BM-MSCs (intrathecally) or NPs (SPC-01 cells or iPSC-NPs, both intraspinally), or saline. The rats were assessed for their locomotor skills (BBB, flat beam test, and rotarod). Morphometric analyses of spared white and gray matter, axonal sprouting, and glial scar formation, as well as qPCR and Luminex assay, were conducted to detect endogenous gene expression, while inflammatory cytokine levels were performed to evaluate the host tissue response to stem cell therapy. The highest locomotor recovery was observed in iPSC-NP-grafted animals, which also displayed the highest amount of preserved white and gray matter. Grafted iPSC-NPs and SPC-01 cells significantly increased the number of growth-associated protein 43 (GAP43(+)) axons, reduced astrogliosis, downregulated Casp3 expression, and increased IL-6 and IL-12 levels. hMSCs transiently decreased levels of inflammatory IL-2 and TNF-alpha. These findings correlate with the short survival of hMSCs, while NPs survived for 2 months and matured slowly into glia-and tissue-specific neuronal precursors. SPC-01 cells differentiated more in astroglial phenotypes with a dense structure of the implant, whereas iPSC-NPs displayed a more neuronal phenotype with a loose structure of the graft. We concluded that the BBB scores of iPSC-NP- and hMSC-injected rats were superior to the SPC-01-treated group. The iPSC-NP treatment of spinal cord injury (SCI) provided the highest recovery of locomotor function due to robust graft survival and its effect on tissue sparing, reduction of glial scarring, and increased axonal sprouting. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2018
Počet záznamů: 1