Počet záznamů: 1
Manipulating Wnt signaling at different subcellular levels affects the fate of neonatal neural stem/progenitor cells
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SYSNO ASEP 0472080 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Manipulating Wnt signaling at different subcellular levels affects the fate of neonatal neural stem/progenitor cells Tvůrce(i) Kriska, J. (CZ)
Honsa, P. (CZ)
Dzamba, D. (CZ)
Butenko, O. (CZ)
Kolenicova, D. (CZ)
Janečková, Lucie (UMG-J)
Nahácka, Zuzana (UMG-J)
Anděra, Ladislav (UMG-J) RID
Kozmik, Zbyněk (UMG-J) RID
Taketo, M.M. (JP)
Kořínek, Vladimír (UMG-J) RID
Anderova, M. (CZ)Celkový počet autorů 12 Zdroj.dok. Brain Research. - : Elsevier - ISSN 0006-8993
Roč. 1651, podzim (2016), s. 73-87Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova beta-catenin signaling ; neonatal mouse ; neurogenesis ; gliogenesis ; patch-clamp technique ; lon channel Vědní obor RIV EB - Genetika a molekulární biologie CEP GBP304/12/G069 GA ČR - Grantová agentura ČR Institucionální podpora UMG-J - RVO:68378050 UT WOS 000387527100009 DOI 10.1016/j.brainres.2016.09.026 Anotace The canonical Wnt signaling pathway plays an important role in embryogenesis, and the establishment of neurogenic niches. It is involved in proliferation and differentiation of neural progenitors, since elevated Wnt/beta-catenin signaling promotes differentiation of neural stem/progenitor cells (NS/PCs1) towards neuroblasts. Nevertheless, it remains elusive how the differentiation program of neural progenitors is influenced by the Wnt signaling output. Using transgenic mouse models, we found that in vitro activation of Wnt signaling resulted in higher expression of beta-catenin protein and Wnt/beta-catenin target genes, while Wnt signaling inhibition resulted in the reverse effect. Within differentiated cells, we identified three electrophysiologically and immunocytochemically distinct cell types, whose incidence was markedly affected by the Wnt signaling output. Activation of the pathway suppressed gliogenesis, and promoted differentiation of NS/PCs towards a neuronal phenotype, while its inhibition led to suppressed neurogenesis and increased counts of cells of glial phenotype. Moreover, Wnt signaling hyper-activation resulted in an increased incidence of cells expressing outwardly rectifying K+ currents, together with inwardly rectifying Na+ currents, a typical current pattern of immature neurons, while blocking the pathway led to the opposite effect. Taken together, our data indicate that the Wnt signaling pathway orchestrates neonatal NS/PCs differentiation towards cells with neuronal characteristics, which might be important for nervous tissue regeneration during central nervous system disorders. Furthermore, the transgenic mouse strains used in this study may serve as a convenient tool to manipulate beta-catenin-dependent signaling in neural progenitors in the neonatal brain. (C) 2016 Elsevier B.V. All rights reserved. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2017
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