TATAA courses – 3 days Hands-on qPCR

0468622 - BTÚ 2017 RIV eng U - Uspořádání akce
Kubista, Mikael - Korenková, Vlasta - Šindelka, Radek - Tománková, Silvie - Valihrach, Lukáš - Švec, David - Langerová, Lucie - Androvič, Peter
TATAA courses – 3 days Hands-on qPCR.
[Vestec, BTÚ, 18.04.2016-20.04.2016, (W-WRD 11/5)]
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: qPCR * biostatistic * reverese transcription
Kód oboru RIV: EB - Genetika a molekulární biologie

Day 1 - This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains: Basic PCR theory. The theory of real-time PCR. Applications and possibilities of qPCR. Comparison of qPCR with regular PCR. Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc). Different instrument platforms and their typical uses. Primer design. The problem of primer-dimer formation and how to minimize them. Probe design. Experimental design and optimization. Basic data handling and analysis.
Day 2 - Introduction to quantification principles. Quantification strategies, uses and limitations. Calculations using different relative quantification methods. Strategies for normalization of qPCR data. In situ calibration for compensation of inhibition in samples. Absolute quantification.
Day 3 - Sample Preparation (Extraction of RNA and DNA). Principles of RT. Priming methods for RT. RT optimization. The MIQE guidelines. Group discussion, bring your own questions.
Trvalý link: http://hdl.handle.net/11104/0266678