Počet záznamů: 1
Simultaneous detection of multiple targets for ultrastructural immunocytochemistry
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SYSNO ASEP 0434293 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Simultaneous detection of multiple targets for ultrastructural immunocytochemistry Tvůrce(i) Philimonenko, Vlada (UMG-J)
Filimonenko, Anatolij (UMG-J)
Šloufová, I. (CZ)
Hrubý, Martin (UMCH-V) RID, ORCID
Novotný, F. (CZ)
Halbhuber, Z. (CZ)
Krivjanská, M. (CZ)
Nebesářová, Jana (BC-A) RID, ORCID
Šlouf, Miroslav (UMCH-V) RID, ORCID
Hozák, Pavel (UMG-J) RID, ORCIDZdroj.dok. Histochemistry and Cell Biology. - : Springer - ISSN 0948-6143
Roč. 141, č. 3 (2014), s. 229-239Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Immunolabeling ; Metal nanoparticles ; Electron microscopy ; Cell nucleus ; Ultrastructure ; Phosphatidylinositol-4,5-Bisphosphate (PIP2) Vědní obor RIV EB - Genetika a molekulární biologie CEP KAN200520704 GA AV ČR - Akademie věd TE01020118 GA TA ČR - Technologická agentura ČR GAP305/11/2232 GA ČR - Grantová agentura ČR LD12063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMCH-V - RVO:61389013 ; BC-A - RVO:60077344 ; UMG-J - RVO:68378050 CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000332082300001 DOI 10.1007/s00418-013-1178-6 Anotace Simultaneous detection of biological molecules by means of indirect immunolabeling provides valuable information about their localization in cellular compartments and their possible interactions in macromolecular complexes. While fluorescent microscopy allows for simultaneous detection of multiple antigens, the sensitive electron microscopy immunodetection is limited to only two antigens. In order to overcome this limitation, we prepared a set of novel, shape-coded metal nanoparticles readily discernible in transmission electron microscopy which can be conjugated to antibodies or other bioreactive molecules. With the use of novel nanoparticles, various combinations with commercial gold nanoparticles can be made to obtain a set for simultaneous labeling. For the first time in ultrastructural histochemistry, up to five molecular targets can be identified simultaneously. We demonstrate the usefulness of the method by mapping of the localization of nuclear lipid phosphatidylinositol-4,5-bisphosphate together with four other molecules crucial for genome function, which proves its suitability for a wide range of biomedical applications. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2015
Počet záznamů: 1