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The effect of tetrabromobisphenol a on protamination and DNA quality of mouse sperm
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SYSNO ASEP 0428712 Druh ASEP K - Konferenční příspěvek (lokální konf.) Zařazení RIV Záznam nebyl označen do RIV Název The effect of tetrabromobisphenol a on protamination and DNA quality of mouse sperm Tvůrce(i) Žatecká, Eva (BTO-N)
Castillo, J. (ES)
Elzeinová, Fatima (BTO-N) RID
Kubátová, Alena (BTO-N) RID
Děd, Lukáš (BTO-N) RID
Pěknicová, Jana (BTO-N) RID
Oliva, R. (ES)Celkový počet autorů 7 Zdroj.dok. Book of abstracts XXth Symposium of Biology and Immunology of Reproduction with international participation. - Praha : Biotechnologický ústav AVČR, v.v.i, 2014 / Pěknicová Jana
S. 1-82Poč.str. 1 s. Akce XXth Symposium of Biology and Immunology of Reproduction with international participation Datum konání 22.05.2014-24.05.2014 Místo konání Třešť Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova tetrabromobisphenol A ; protamine ; spermatozoa Vědní obor RIV EB - Genetika a molekulární biologie CEP P503/12/1834 GA ČR - Grantová agentura ČR CZ1.05/1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BTO-N - RVO:86652036 CEZ AV0Z50520701 - BTO-N (2007-2013) Anotace Tetrabromobisphenol (TBBPA) is a widely used brominated flame retardant, currently its consumption is 210,000 tons / year and is still growing. In our previous multigenerational in vivo study we have demonstrated that TBBPA is able to induce apoptosis of testicular cells and changes in the expression of genes important for proper spermatogenesis. However the potential effect of TBBPA on epidydimal spermatozoa had not yet been investigated. Therefore, we performed further study to evaluate the effect of on sperm DNA integrity and on the protamines as the major nuclear proteins. C57Bl/6J mice pups (n=10) were exposed to TBBPA (experimental group) during the gestation, lactation, pre-pubertal and pubertal periods up to the age of 70 days and compared control mice pups (n= 10) which were not exposed. Our results demonstrate that TBBPA treatment results in a significantly decreased P1/P2 ratio, increased total protamine/DNA ratio and increased DNA fragmentation observed between TBBPA and control mice, respectively. Protamines have recently been connected to the epigenetic marking of sperm chromatin in human and mouse spermatozoa. Thus, our findings suggest that TBBPA exposure, in addition to result in increased sperm DNA damage, may also alter the epigenetic marking of sperm chromatin. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2015
Počet záznamů: 1