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FBH1 Helicase Disrupts RAD51 Filaments in Vitro and Modulates Homologous Recombination in Mammalian Cells
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SYSNO ASEP 0423311 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název FBH1 Helicase Disrupts RAD51 Filaments in Vitro and Modulates Homologous Recombination in Mammalian Cells Tvůrce(i) Šimandlová, Jitka (UMG-J)
Zagelbaum, J. (US)
Payne, M.J. (GB)
Chu, W.K. (US)
Shevelev, Igor (UMG-J)
Hanada, K. (GB)
Chatterjee, S. (US)
Reid, D.A. (US)
Liu, Y. (DE)
Janščák, Pavel (UMG-J) RID
Rothenberg, E. (US)
Hickson, I.D. (GB)Zdroj.dok. Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 288, č. 47 (2013), s. 34168-34180Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova DNA damage ; DNA helicase ; DNA recombination ; DNA repair ; DNA replication Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP305/10/0281 GA ČR - Grantová agentura ČR Institucionální podpora UMG-J - RVO:68378050 UT WOS 000327250200060 DOI 10.1074/jbc.M113.484493 Anotace Background: Homologous recombination is regulated both positively and negatively in eukaryotic cells to suppress genomic instability. Results: FBH1 can disrupt RAD51 filaments in vitro and suppresses formation of spontaneous RAD51 foci in mammalian cells. In cells defective for FBH1, hyper-recombination is observed. Conclusion: FBH1 is a negative regulator of homologous recombination. Significance: RAD51 activity must be carefully controlled to preserve genomic integrity. Efficient repair of DNA double strand breaks and interstrand cross-links requires the homologous recombination (HR) pathway, a potentially error-free process that utilizes a homologous sequence as a repair template. A key player in HR is RAD51, the eukaryotic ortholog of bacterial RecA protein. RAD51 can polymerize on DNA to form a nucleoprotein filament that facilitates both the search for the homologous DNA sequences and the subsequent DNA strand invasion required to initiate HR. Because of its pivotal role in HR, RAD51 is subject to numerous positive and negative regulatory influences. Using a combination of molecular genetic, biochemical, and single-molecule biophysical techniques, we provide mechanistic insight into the mode of action of the FBH1 helicase as a regulator of RAD51-dependent HR in mammalian cells. We show that FBH1 binds directly to RAD51 and is able to disrupt RAD51 filaments on DNA through its ssDNA translocase function. Consistent with this, a mutant mouse embryonic stem cell line with a deletion in the FBH1 helicase domain fails to limit RAD51 chromatin association and shows hyper-recombination. Our data are consistent with FBH1 restraining RAD51 DNA binding under unperturbed growth conditions to prevent unwanted or unscheduled DNA recombination. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2014
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