Počet záznamů: 1
Mechanistic Study of 17 alpha-Ethinylestradiol Biodegradation by Pleurotus ostreatus: Tracking of Extracelullar and Intracelullar Degradation Mechanisms
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SYSNO ASEP 0388819 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Mechanistic Study of 17 alpha-Ethinylestradiol Biodegradation by Pleurotus ostreatus: Tracking of Extracelullar and Intracelullar Degradation Mechanisms Tvůrce(i) Křesinová, Zdena (MBU-M) RID
Moeder, M. (DE)
Ezechiáš, Martin (MBU-M) RID
Svobodová, Kateřina (MBU-M) RID
Cajthaml, Tomáš (MBU-M) RID, ORCIDZdroj.dok. Environmental Science and Technology - ISSN 0013-936X
Roč. 46, č. 24 (2012), s. 13377-13385Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova WHITE-ROT FUNGI ; ENDOCRINE-DISRUPTING CHEMICALS ; PERSONAL CARE PRODUCTS Vědní obor RIV EE - Mikrobiologie, virologie CEP TA01020804 GA TA ČR - Technologická agentura ČR GAP503/10/0408 GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000312432200044 DOI 10.1021/es3029507 Anotace The white rot fungus Pleurotus ostreatus is able to completely remove the synthetic hormone 17 alpha-ethinylestradiol (EE2, 200 mu g in 20 mL) from a liquid complex or mineral medium in 3 or 14 days, respectively. Its efficiency has also been documented in the removal of estrogenic activity that correlated with the EE2 degradation. A set of in vitro experiments using various cellular and enzyme fractions has been performed and the results showed that EE2 was degraded by isolated laccase (about 90% within 24 h). The degradation was also tested with concentrated extracellular liquid where degradation reached 50% mainly due to the laccase activity; however, after a supplementation with H2O2 and Mn2+, residual manganese-dependent peroxidase activities (40 times lower than Lac) raised the degradation to 100%. Moreover, the intracellular fraction and also laccase-like activity associated with fungal mycelium were found to be efficient in the degradation too Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2013
Počet záznamů: 1