Počet záznamů: 1
Nanomedicine and Drug Delivery
- 1.
SYSNO ASEP 0381465 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Polymer-modified gene delivery vectors retargeted with recombinant proteins Tvůrce(i) Pola, Robert (UMCH-V) RID, ORCID
Pechar, Michal (UMCH-V) RID, ORCID
Ulbrich, Karel (UMCH-V) RID
Carlisle, R. C. (GB)
Willemsen, R. A. (NL)
Seymour, L. W. (GB)Zdroj.dok. Nanomedicine and Drug Delivery, Volume 1. - Oakville : Apple Academic Press, 2012 / Sebastian M. ; Ninan N. ; Haghi A. K. - ISBN 978-1-926895-17-8 Rozsah stran s. 33-38 Poč.str. 6 s. Poč.str.knihy 320 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CA - Kanada Klíč. slova bungarotoxin ; poly(ethylene glycol) ; prostate-specific membrane antigen Vědní obor RIV CD - Makromolekulární chemie CEP GA203/08/0543 GA ČR - Grantová agentura ČR 1M0505 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z40500505 - UMCH-V (2005-2011) Anotace We have prepared and characterized a multivalent reactive N-(2-hydroxypropyl)methacrylamide copolymer (PHPMA) bearing bungarotoxin-binding peptide (BTXbp). The polymer was used for covalent surface modification of adenoviral vectors containing luciferase reporter gene (Ad). The peptide BTXbp is known to have extremely high binding affinity to bungarotoxin (BTX) –a protein strongly binding to acetylcholine receptors. A recombinant protein consisting of antiPSMA antibody scFv fragment and BTX binding site (BTX-scFv) was used for retargeting of the polymer-modified Ad to prostate-specific membrane antigen (PSMA) receptors. While the polymer-modified Ad exhibited approximately 100-fold lower infectivity than the naked virus (in terms of luciferase expression), the addition of BTX-scFv to the polymer-coated Ad led to about 10-fold restoration of luciferase expression in PSMA-positive LNCaP cells. No such increase of transduction activity was observed in PSMA-negative PC3 cells. We have shown that the presented PHPMA-BTXbp/BTX-scFv system can be used as a universal tool for a receptor-specific viral gene therapy. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2013 Elektronická adresa http://www.appleacademicpress.com/title.php?id=9781926895178
Počet záznamů: 1